矮晚柚的组织培养与快速繁殖

Tissue culture and rapid propagation of dwarf late pomelo

关键词:  矮晚柚  丛生芽诱导  组织培养  快速繁殖

Key words:  dwarf late pomelo  cespitose buds induction  tissue culture  rapid proliferation

摘要:

以矮晚柚种子萌发的无菌苗为实验材料，利用茎尖和上胚轴诱导丛生芽的发生，利用丛生芽获得再生植株。实验表明：矮晚柚成熟和未成熟种子在1/2MS、MS上均能萌发，萌发率最高可达96%，成熟种子萌发的无菌苗更利于后期的分化。最适外植体为无菌苗的上胚轴，筛选出丛生芽最佳增殖培养方案为MS+6 BA 2.0 mg•L 1+NAA 0.1 mg•L 1+蔗糖40 g•L 1+靠近茎尖上胚轴，最高增殖系数达8.4，最佳生根培养基为1/2MS+NAA 0.2 mg•L 1+IBA 0.2 mg•L 1+活性炭0.2 g•L 1，生根率达90%以上。移栽至蛭石+珍珠岩+营养土（1∶1∶2）的营养砵上，成活率可达80%。

Abstract:

Aseptic seeding of dwarf late pomelo were used as the materials in the study，We induced cespitose buds from meristem and epicotyl of the seedlings，then regenerated plants from the induced shoots，and results showed that both mature and immature seeds could be germinate on 1/2MS and MS medium，the highest germination rate could reach 96%，and the aseptic seeding from mature seeds were more suitable for the further differentiation. The epicotyl was found to be the optimal explants，the optimal protocol for cespitose buds proliferation was to culutre upicotyl on MS medium 〖JP3〗with 2.0 mg•L 1 6 BA，0.1 mg•L 1 NAA and 40 g•L 1 sucrose，and the achieved multiplication coefficient was 8.4. The optimal rooting medium was 1/2MS medium with 0.2 mg•L 1 NAA，0.2 mg•L 1 IBA 0.2 g•L 1 AC，〖JP2〗from which 90% rooting rate was obtained. After ransplanted to nutrition bowl，80% plants survived.