金线兰种子萌发及其原球茎的增殖培养

Seed germination and the protocorm multiplication of Anoectochilus roxburghii

关键词:  金线兰  种子萌发  原球茎  增殖

Key words:  Anoectochilus roxburghii  seed germination  protocorm  multiplication

摘要:

研究了影响金线兰种子非共生萌发的因素，并应用正交试验研究基本培养基、6 BA、ZT、NAA四种因素对原球茎增殖的作用。结果表明：授粉类型对金线兰种子非共生萌发影响较大，异株异花、同株异花以及同株同花授粉所得的种子的萌发率分别为78.53%、69.62%、39.87%；蒴果成熟度以生长150 d为宜，采收后萌发率可达78.59%；冷藏影响种子的活力，种子的萌发率随冷藏时间的延长而降低；使用次氯酸钠浸泡后的种子与对照相比，其萌发率无明显差异；NAA对原球茎增殖作用显著，适宜于原球茎增殖的培养基为1/2MS+ZT0.5mg/L+NAA1.0mg/L。

Abstract:

Seed germination of Anoectochilus roxburghii in asymbiotie culture and the effects of four factors(culture medium，6 BA，ZT and NAA)on protocorm multiplication through orthogonal design were studied. The results showed that pollination type was an important factor on A.roxburghii seeds' non symbiotic germination，germination ratio of the xenogamy seeds reached 78.53%，and the germination ratio of the geitonogamy seeds reached 69.62%，while the germination ratio of self pollination was only 39.87%. The most suitable maturity of capsule appeared when it grew for 150 days，the germination rate of such seeds could reach 78.59%. The vitality of the seeds were affected by refrigerated storage:the germination rate of the seeds was decreased with the increase of seed cool storage time. There were no significant changes of seed germination rate between seed treatment by 10% NaClO solution and the control. NAA had a significant effect on the proliferation of protocorm，the optimum culture medium for protocorm proliferation was 1/2MS+ZT0.5mg/L+NAA1.0mg/L.