| 摘要: |
| 研究了影响金线兰种子非共生萌发的因素，并应用正交试验研究基本培养基、6 BA、ZT、NAA四种因素对原球茎增殖的作用。结果表明：授粉类型对金线兰种子非共生萌发影响较大，异株异花、同株异花以及同株同花授粉所得的种子的萌发率分别为78.53%、69.62%、39.87%；蒴果成熟度以生长150 d为宜，采收后萌发率可达78.59%；冷藏影响种子的活力，种子的萌发率随冷藏时间的延长而降低；使用次氯酸钠浸泡后的种子与对照相比，其萌发率无明显差异；NAA对原球茎增殖作用显著，适宜于原球茎增殖的培养基为1/2MS+ZT0.5mg/L+NAA1.0mg/L。 |
| 关键词: 金线兰 种子萌发 原球茎 增殖 |
| DOI： |
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| Seed germination and the protocorm multiplication of Anoectochilus roxburghii |
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QIU Yue1，2， GONG Ning1，2*， ZHANG KuiYi1，2
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1.College of Life Sciences， Guizhou Normal University， Guiyang 550001， China;2.2.Guizhou Provincial Key Laboratory for Montain Environment， Guiyang 550001， China
1.College of Life Sciences， Guizhou Normal University， Guiyang 550001， China； 2.Guizhou Provincial Key Laboratory for Montain Environment， Guiyang 550001， China
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| Abstract: |
| Seed germination of Anoectochilus roxburghii in asymbiotie culture and the effects of four factors(culture medium，6 BA，ZT and NAA)on protocorm multiplication through orthogonal design were studied. The results showed that pollination type was an important factor on A.roxburghii seeds' non symbiotic germination，germination ratio of the xenogamy seeds reached 78.53%，and the germination ratio of the geitonogamy seeds reached 69.62%，while the germination ratio of self pollination was only 39.87%. The most suitable maturity of capsule appeared when it grew for 150 days，the germination rate of such seeds could reach 78.59%. The vitality of the seeds were affected by refrigerated storage:the germination rate of the seeds was decreased with the increase of seed cool storage time. There were no significant changes of seed germination rate between seed treatment by 10% NaClO solution and the control. NAA had a significant effect on the proliferation of protocorm，the optimum culture medium for protocorm proliferation was 1/2MS+ZT0.5mg/L+NAA1.0mg/L. |
| Key words: Anoectochilus roxburghii seed germination protocorm multiplication |
