濒危药用植物盘龙参根的显微结构及其内生真菌分布研究

Microstructure and endophytic fungus distribution of the root of endangered and officinal plant Spiranthes sinensis

关键词:  兰科  盘龙参  石蜡切片  光学显微摄像  内生真菌

Key words:  Orchidaceae  Spiranthes sinensis  permanent paraffin-cut section  optical microscope photography  endophytic fungus

摘要:

采用石蜡永久制片和光学显微摄像的方法,对盘龙参根的显微结构及其内生真菌的分布进行了研究。结果表明:盘龙参的根由表皮、皮层和中柱组成,表皮上有根毛,皮层所占根径的比例达70%以上,其内草酸钙针晶散在或成束于皮层细胞中。中柱由18～22个木质部与韧皮部相间排列组成。盘龙参根的皮层细胞和中柱中有内生真菌的分布; 内生真菌由表皮、外皮层侵入到皮层薄壁细胞和中柱的薄壁细胞中,在皮层和中柱中形成菌丝和菌丝结,并扩展成一定的侵染区域,同时在部分皮层细胞中菌丝结已被消化吸收。

Abstract:

Microstructure and distribution of endophytic fungi of the root of Spiranthes sinensis were studied through permanent paraffin-cut section and optical microscoipe photography. The results indicated that the rhizome of S.sinensis consisted of epidermis,cortex and stele. The epidermis had many root hairs,the cortex occupied more than 70% of the diameter of root,in which the cylinder were sometimes observed. Stele were scattered by eighteen to twenty-two xylem and phloem. The hyphae of endophytic fungi were colonized in the cortex and stele. They infected the cortex parenchyma through epidermis and exodermis,and then formed pelotons,and expanded their occupying area of cortex.