水稻花药愈伤组织诱导的多因子正交试验研究

Studies on anther callus induction of rice by orthogonal test

关键词:  水稻  花药  愈伤组织诱导  正交试验

Key words:  rice  anther  callus induction  orthogonal test

摘要:

以密阳46和培矮64/8006杂交F1代的花药为材料，研究两个材料花粉发育时期与叶枕距大小的相关性。结果表明，密阳46在叶枕距5～7 cm时，小孢子大部分处于单核靠边期；培矮64/8006杂交F1代稍迟，叶枕距为7～9 cm时，大部分小孢子才进入单核靠边期。同时通过L9(34)正交试验，研究了培养基类型、激素配比、蔗糖和2，4 D浓度对花药出愈率的影响，筛选出两个品种愈伤组织诱导的最佳培养条件：密阳46为SK3+4 mg•L 1 2，4 D+1 mg•L 1 NAA+0.5 mg•L 1 KT+3%蔗糖，培矮64/8006杂交F1代为合5+2 mg•L 1 2，4 D+1 mg•L 1 NAA+0.5 mg•L 1 KT+6%蔗糖。

Abstract:

Using the anther of Milyang 46 and the F1 hybrids of Pei’ai 64/8006，the correlation of pollen development with the distance of the pulvinus was studied. The results showed that most of the microspores were in the late uninucleate stage when the distance of the pulvinus was 5-7 cm for Milyang 46；while it was 7-9 cm for the F1 hybrids of Pei’ai 64/8006. Besides，the effects on anther callus induction rate of the types of medium，the ration of plant hormones，the concentration of the sucrose and 2，4 D were investigated by using orthogonal tests L9(34)，and the best conditions for callus induction of the two accessions were selected:the Milyang 46 was SK3+4 mg•L 1 2，4 D+1 mg•L 1 NAA+0.5 mg•L 1 KT+3%sucrose while the F1 hybrids of Pei’ai 64/8006 was HE5+2 mg•L 1 2，4 D+1 mg•L 1 NAA+0.5 mg•L 1 KT +6%sucrose.