观赏凤梨高效离体快繁影响因素的研究

龚明霞1; $22; $23; $24; $21; $21

引用本文:	龚明霞1，张志胜2，黎杨辉3，方锋学4，何铁光1，董文斌1.观赏凤梨高效离体快繁影响因素的研究[J].广西植物,2011,(5):684-689.[点击复制]
	GONG Ming Xia1， ZHANG Zhi Sheng2， LI Yang Hui3， FANG Feng Xue4， HE Tie Guang1， DONG Wen Bin1.Influencing factors of efficient and rapid propagation in vitro of Bromeliads[J].Guihaia,2011,(5):684-689.[点击复制]

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观赏凤梨高效离体快繁影响因素的研究
龚明霞1，张志胜2，黎杨辉3，方锋学4，何铁光1，董文斌1
1．广西农科院蔬菜研究所，南宁 530007;2．华南农业大学广东省植物分子育种重点实验室，广州 510642;3．广州花卉研究中心，广州 510360;4．广西甘蔗研究所，南宁 530007

摘要:

以侧芽为外植体材料，对其组织培养的各个阶段进行了研究。结果表明：品种对外植体的污染率、褐变率、芽诱导率及丛生芽增殖倍数均有显著影响；外源激素的种类和浓度在芽诱导分化、增殖、生根等各个阶段都是主要的影响因素；外植体在1/2MS＋NAA0.2 mg•L 1＋6 BA2 mg•L 1上，48 d后芽开始分化，诱导率为40%，平均芽分化数为6个，诱导分化效果最好；芽增殖培养阶段，若培养基中仅含6 BA，且浓度在0～4 mg•L 1之间，芽增殖倍数随其浓度增大而增大；若培养基中仅含NAA，且其浓度为0.2 mg•L 1时，增殖倍数最大；培养基中同时添加6 BA和NAA，比单独添加6 BA或NAA时，芽的增殖效果好，且在6 BA3 mg•L 1+NAA0.5 mg•L 1的MS培养基上，G.dissitisflora和G.‘Claret’增殖倍数最大，分别为5.24和3.84；在任何相同的培养基上，G.dissitisflora 的增殖倍数显著高于G.‘Claret’；小苗在含有NAA 0.5 mg•L 1＋IBA05 mg•L 1的生根培养基上生长，生根率可达91.03%，平均根数为5.3条/株，生根效果较好。

关键词: 观赏凤梨离体快繁影响因素

DOI：

分类号:Q943

基金项目:

Influencing factors of efficient and rapid propagation in vitro of Bromeliads

GONG MingXia1， ZHANG ZhiSheng2， LI YangHui3， FANG FengXue4， HE TieGuang1， DONG WenBin1

1.Institute of Vegetable Research，Guangxi Academy of Agricultural Sciences， Nanning 530007， China;2.2.Guangdong Provincial Key Laboratory of Plant Molecular Breeding，South China Agricultural University， Guangzhou 510642， China;3.Guangzhou Flower Research Center， Guangzhou 510360， China;4.Guangxi Research Institute of Chemical Industry， Nanning 530001， China 1.Institute of Vegetable Research，Guangxi Academy of Agricultural Sciences， Nanning 530007， China； 2.Guangdong Provincial Key Laboratory of Plant Molecular Breeding，South China Agricultural University， Guangzhou 510642， China； 3.Guangzhou Flower Research Center， Guangzhou 510360， China； 4.Guangxi Research Institute of Chemical Industry， Nanning 530001， China

Abstract:

This paper，every stage was studied by using lateral buds as explants. The results showed that the variety had remarkable influence on the contamination rate and the browning rate of explants，and the induction rate and the proliferation multiple of tufty buds. The variety and concentration of exogenous hormone were the major influencing factors in every stage such as the bud induction, proliferation and the root induction. In the bud induction stage，it got the best result of induction differentiation，which occured after 48 days with 40% induction rate and average six buds per explant when they were cultured on 1/2MS basal media supplemented with NAA 0.2 mg•L 1 and 6 BA 2 mg•L 1. In the bud proliferation stage，when the concentration of 6 BA solely supplemented in the media between 0-4 mg•L 1 was higher，the proliferation multiple was higher. When the concentration of NAA solely supplemented in the media was 0.2 mg•L 1，the proliferation multiple was the highest. It got better proliferation on the media supplemented with both 6 BA and NAA than that on the media supplemented with 6 BA or NAA solely. On MS media supplemented with 3 mg•L 1 6 BA and 0.5 mg•L 1NAA，the proliferation multiple of G.dissitisflora and G.‘Claret’both got to the top，which was 5.24，3.84 respectively. On any the same media，the proliferation multiple of G.dissitisflora was remarkable higher than that of G.‘Claret’. It was better to root when the plantlets grew on 1/2MS media containing 0.5 mg•L 1 NAA and 0.5 mg•L 1 IBA，and the rooting rate could reach 91.03%，and the average number of the roots was 5.3 piece per plant.

Key words: Bromeliads rapid propagation in vitro influencing factors