板栗种子发育期间ABA等生理指标与脱水耐性的相关性研究

Study on the correlation between physiological indexes as ABA， etc and desiccation tolerance in developing chestnut seeds

关键词:  板栗  脱水耐性  顽拗性种子  ABA  淀粉

Key words:  castanea mollisima  desiccation tolerance  recalcitrant seeds  ABA  starch

摘要:

对板栗种子发育期间胚轴和子叶中部分生理指标的变化以及它们与板栗种子脱水耐性的相关性进行研究。结果表明：随着板栗种子不断发育，在花后80 d胚轴和子叶中ABA、淀粉、可溶性蛋白质量分数同步达到最大值，可溶性糖质量分数达到最低，此时板栗种子的脱水耐性最强，可确定为板栗的最佳采收期。另外，通过相关分析可知，板栗种子在发育期间脱水耐性的获得主要与胚轴中物质的变化有关，且与淀粉质量分数呈极显著正相关，与ABA、可溶性蛋白质量分数呈高度正相关，与可溶性糖质量分数呈中度负相关。由此得出，根据淀粉质量分数的变化可以初步推测出板栗种子最大脱水耐性时期。

Abstract:

The partial physiological indexes of embryonic axes and cotyledons in the developing chestnut seeds，and the correlation between them and the desiccation tolerance were studied. Experimental results showed that with the development of chestnut seeds，the contents of ABA，starch and soluble proteins of embryonic axes and cotyledons on the 80 days after anthesis reached synchronously the maximum，the content of soluble sugars of embryonic axes and cotyledons on the 80 days after anthesis was the minimum. At the moment the desiccation tolerance of chestnut seeds on the 80 days after anthesis was the strongest，so it should be the optimal harvest period for chestnut. In addition，by correlation analysis，the acquisition of desiccation tolerance in the developing chestnut seed was related to the changes of matter in embryonic axes. It was significantly correlative with starch and the contents of ABA and soluble proteins，and was negatively correlative with the contents of soluble sugars. Therefore，the period of maximum desiccation tolerance in chestnut seeds could be inferred by the change of starch.