桑树肌动蛋白actin基因全长序列的克隆与分析

Cloning and sequence analysis of full length of mulberry (Morus alba) actin gene

关键词:  桑树  肌动蛋白  染色体步移  克隆  序列分析

Key words:  Morus alba  actin  chromosome walking  cloning  sequence analysis

摘要:

肌动蛋白在植物的各种生理活动中起着重要作用，是研究基因表达与调控模式的内标参考。通过染色体步移方法获得了桑树肌动蛋白actin基因1 612 bp的序列，该基因CDS长1 312 bp（GenBank登录号：HM623866），编码377个氨基酸残基，与水稻、葡萄等的同源基因的序列一致性在90%以上。基因内含子的数目及其在基因组上的位置也与水稻、葡萄等的相似。对来自不同物种的24个肌动蛋白基因进行聚类分析的结果显示，基因被分为Class Ⅰ和Class Ⅱ两个明显的亚群。

Abstract:

As a standard reference model of gene expression and regulation，actins play important roles in plants. In this study，the full length 1 612 bp of actin gene from mulberry was cloned by means of chromosome walking. Its CDS was 1 312 bp in length，encoding 377 amino acid residues. Homology analysis of the deduced amino acids showed above 90% identity with the actins from rice and grape，et al. The number and site of introns was conservative with rice and grape，too. Cluster analysis of actins from 24 species indicated that these genes were divided into two subgroups of Class I and Class Ⅱ.