金钗石斛生物碱的组织化学定位研究

Histochemical localization of alkaloid in Dendrobium nobile

关键词:  金钗石斛  生物碱  组织化学定位

Key words:  Dendrobium nobile  alkaloid  histochemical localization

摘要:

利用组织化学定位方法对金钗石斛不同生长年限和不同营养器官的生物碱分布和积累进行比较分析。结果表明,金钗石斛茎生物碱主要分布于基本组织,三年生金钗石斛的生物碱含量高于一年生和二年生金钗石斛。金钗石斛根部生物碱含量同样较多,主要分布于皮层薄壁细胞中; 金钗石斛叶片生物碱含量较低,存在于上下表皮细胞中,与铁皮石斛和铜皮石斛完全不同。说明三年生金钗石斛作为最适宜采收期,而且金钗石斛根部具有很好的开发利用价值。金钗石斛叶片表皮细胞中呈现生物碱的原因尚未清楚,需要进一步研究。

Abstract:

The alkaloid contents in Dendrobium nobile of different developmental stages and organs were analyzed by using histochemical localization method. The results showed that the alkaloid was located in the fundamental tissue of stem,cortex of root and epidermis of leaf. Furthermore,the alkaloid contents of D.nobile of three-year-old were higher than that of one-year-old and two-year-old. And in different organs of this species,the alkaloid content in stem was almost equal to that in root,while it was general higher than that in leaf. These results implied that the best collection period of D.nobile was three-year old,and the root of D.nobile had a good development and utilizable value. It is necessary to further study why epidermis of leaf of D.nobile had alkaloid.