基于ISSR分子标记的枇杷材料亲缘关系分析

Relationship identification of a loquat variant based on Inter-Simple Sequence Repeat(ISSR)

关键词:  川早枇杷  ISSR-PCR  变异  亲缘关系

Key words:  Chuanzao loquat  ISSR-PCR  variation  genetic relationship

摘要:

为了判断川早枇杷与其他枇杷材料间的亲缘关系,探讨ISSR分析鉴定枇杷变异的有效性。以川早枇杷等13份枇杷品种(品系)为材料,通过DNA提取、ISSR-PCR反应体系建立、引物筛选及PCR扩增,使用Quantity One软件对扩增条带进行统计分析,计算遗传相似系数,并进行UPGMA聚类分析。结果表明:用14条ISSR引物进行扩增,共获得165条带,条带长度为300～1 500 bp; 日本茂木与川早枇杷的遗传相似系数最高,为0.7920,早钟六号和晚钟与川早枇杷的遗传相似系数分别为0.7717和0.7444,当遗传相似系数为0.7160时,13份枇杷材料可以被分为4类。因此,相对于早钟六号,川早枇杷材料在遗传物质上发生了变异,具备成为枇杷新品种的条件。

Abstract:

This research was aimed at analyzing the special early character of 13 loquats,including Chuanzao loquat,and the possibility of judging loquat variation by ISSR,which was a new bud mutant line. To make UPGMA(Unweighted Pairgroup Method and Arithetic Average)analysis and calculate genetic similarity,13 loquats were used as materials to extract total DNA,Optimize ISSR System,screen primer and PCR,and detected bands with Quantity One(a software). With 14 ISSR primers,165 bands were found,the length of which was 300-1 500 bp; the genetic similarity between Chuanzao loquat and Maomu was 0.7920,the genetic similarity between Chuanzao loquat,Zaozhong 6 and Wanzhong were 0.7717 and 0.7444 respectively. 13 loquats could be separated into 4 species when the genetic similarity was 0.7160. The Chuanzao loquat showed genetic changes,which meant it colud be a new variety.