| 引用本文: | 张春艳, 王 义, 孙春玉, 王康宇, 张美萍.吉粳88高效再生体系的建立[J].广西植物,2013,(6):865-869.[点击复制] |
| ZHANG Chun-Yan, WANG Yi, SUN Chun-Yu,
WANG Kang-Yu, ZHANG Mei-Ping.Establishment of an efficient regeneration system of Jijing88[J].Guihaia,2013,(6):865-869.[点击复制] |
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| 摘要: |
| 以吉粳88为材料,通过对不同培养基和激素的筛选,确定最佳的再生体系。结果表明:NMB培养基为吉粳88最适的愈伤组织诱导培养基。通过L9(33)正交实验研究最适激素浓度配比,筛选出吉粳88愈伤组织诱导的最佳激素配比为2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.10 mg/L NAA。在添加2.0 mg/L 6-BA+0.5 mg/L NAA的NMB基本培养基条件下,吉粳88的分化率最高,成培苗率也最高。 |
| 关键词: 吉粳88 培养基 诱导率 分化率 成苗率 |
| DOI：10.3969/j.issn.1000-3142.2013.06.026 |
| 分类号:S511 |
| 基金项目: |
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| Establishment of an efficient regeneration system of Jijing88 |
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ZHANG Chun-Yan, WANG Yi, SUN Chun-Yu,
WANG Kang-Yu, ZHANG Mei-Ping*
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Jilin Agricultural University, Changchun 130118, China
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| Abstract: |
| Jijing 88 was used as the research material and different media and hormone combinations were selected,in order to obtain its optimal in-vitro regeneration system. The results showed that NMB medium was the most suitable medium for rice callus induction among the 5 medium. The most suitable plant hormone ratio was investigated by using orthogonal test L9(33),and the induction rate was the highest when 2.0 mg/L 2,4-D,0.2 mg/L 6-BA,0.10 mg/L NAA were added. The differentiation rate and regeneration rate were the highest when 2.0 mg/L6-BA,0.5 mg/L NAA were added. |
| Key words: Jijing 88 culture medium induction rate differentiation rate regeneration rate |
