巴西橡胶树鲨烯合酶基因的克隆与序列分析

张志平; 仇 键; 杨文凤; 魏 芳; 校现周

引用本文:	张志平, 仇键, 杨文凤, 魏芳, 校现周.巴西橡胶树鲨烯合酶基因的克隆与序列分析[J].广西植物,2014,(2):235-241.[点击复制]
	ZHANG Zhi-Ping, Qiu Jian, YANG Wen-Feng, WEI Fang, XIAO Xian-Zhou.Cloning and bioinformatics analysis of squalene synthase gene(SQS)from Hevea brasiliensis[J].Guihaia,2014,(2):235-241.[点击复制]

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巴西橡胶树鲨烯合酶基因的克隆与序列分析
张志平, 仇键, 杨文凤, 魏芳, 校现周
1. 海南大学园艺园林学院, 海南儋州 571737;2. 中国热带农业科学院橡胶研究所, 海南儋州 571737

摘要:

鲨烯合酶(SQS)是植物甾醇和三萜化合物生物合成途径中的关键酶。以巴西橡胶树为试验材料,提取胶乳总RNA,利用RT-PCR以及RACE的方法克隆橡胶树鲨烯合酶cDNA编码区片段,并进行序列分析。结果表明:橡胶树鲨烯合酶cDNA编码区为1 239 bp,编码413个氨基酸,命名为HbSQS。荧光定量分析表明鲨烯合酶基因在不同组织里表达水平存在明显差异,且受乙烯调控。

关键词: 橡胶树鲨烯合酶序列分析

DOI：10.3969/j.issn.1000-3142.2014.02.019

分类号:Q781

基金项目:

Cloning and bioinformatics analysis of squalene synthase gene(SQS)from Hevea brasiliensis

ZHANG Zhi-Ping^1,2, Qiu Jian², YANG Wen-Feng², WEI Fang², XIAO Xian-Zhou^2*

1. College of Horticulture and Landscape Architecture, Hainan University, Danzhou 571737, China;2. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Abstract:

Squalene synthase(SQS)is a key enzyme in plant terpenoid biosynthetic pathway. The total RNA were extracted from Hevea brasiliensis relax,and the cDNA of squalene synthase was cloned using RT-PCR and RACE strategy. The results showed that the cDNA(named as HbSQS)contained a 1 239 bp open reading frame and encoded a predicted protein of 413 amino acids. SYBR Green I Real Time RT-PCR analysis indicated that the HbSQS was more highly expressed in bark than in leaf. The transcription of HbSQS in latex was induced by ethylene.

Key words: Hevea brasiliensis squalene synthase sequence analysis