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| 引用本文: | 黄琼林, 梁凌玲, 何 瑞, 詹若挺, 陈蔚文.应用matK基因鉴别青天葵及其常见混伪品[J].广西植物,2014,(3):299-303.[点击复制] |
| HUANG Qiong-Lin, LIANG Ling-Ling, HE Rui,
ZHAN Ruo-Ting, CHEN Wei-Wen.Apply matK gene to identify Nervilia fordii and its common adulterants[J].Guihaia,2014,(3):299-303.[点击复制] |
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| 应用matK基因鉴别青天葵及其常见混伪品 |
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黄琼林, 梁凌玲, 何 瑞, 詹若挺, 陈蔚文*
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广州中医药大学 中药资源科学与工程研究中心 岭南中药资源教育部重点实验室, 广州 510006
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| 摘要: |
| 分析了青天葵及其混伪品matK序列,以期在分子水平建立青天葵及其常见混伪品的鉴别方法。采用一对通用引物对matK基因进行PCR扩增并测序,所得序列用DNAMAN、MEGA等软件进行分析。获得青天葵及其混伪品的matK基因序列长度为587 bp,平均GC含量为32.8%。青天葵的种内遗传距离为0,与混伪品种间遗传距离范围为0.016～0.375。基于matK基因序列构建的NJ聚类树能明显地区别青天葵及其混伪品。因此,应用matK基因序列可以有效鉴别青天葵及其混伪品。 |
| 关键词: 青天葵 matK基因 混伪品 |
| DOI：10.3969/j.issn.1000-3142.2014.03.004 |
| 分类号:Q948 |
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| Apply matK gene to identify Nervilia fordii and its common adulterants |
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HUANG Qiong-Lin, LIANG Ling-Ling, HE Rui,
ZHAN Ruo-Ting, CHEN Wei-Wen*
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Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Traditional Chinese Medicine),
Ministry of Education, Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou
University of Traditional Chinese Medicine, Guangzhou 510006, China
Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Traditional Chinese Medicine)
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Ministry of Education, Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou
University of Traditional Chinese Medicine, Guangzhou 510006, China
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| Abstract: |
| In order to establish the novel method on distinguishing Nervilia fordii and its common adulterants,matK genes from N. fordii and its adulterants were amplified and sequenced using a pair of universal primes. The results revealed that matK genes acquired from N. fordii and its adulterants contained 587 base pairs,and average GC content was 32.8%. The intraspecific distances among N. fordii were 0,whereas the interspecific distance between N. fordii and its adulterants ranged from 0.016 to 0.375. The clustering tree according to Neighbor Joining method and Kimura 2-Parameter model discriminated N. fordii and its adulterants obviously. matK gene is suggested to be an effective biomarker to identify N. fordii and its common adulterants. |
| Key words: Nervilia fordii matK gene adulterant |
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