大叶桉叶水浸提液成分分析

Composition analysis on water leaching solution of Eucalyptus robusta leaf

关键词:  大叶桉  水浸提液  GC-MS  成分分析

Key words:  Eucalyptus robusta  water leaching solution  GC-MS  component analysis

摘要:

为进一步明确大叶桉的化学成分,对大叶桉叶水浸提液分别用不同极性的有机溶剂石油醚、乙酸乙酯和正丁醇进行萃取,对各萃取相进行GC-MS分析。结果表明:大叶桉叶水浸提液共含有37种化合物,其中,石油醚萃取相中含有20种,主成分为草酸丁基异己酯(37.24%); 乙酸乙酯萃取相中含有16种,主成分为2,2-二亚甲基双[6-(1,1-二甲基乙基-4-甲基)]-苯酚(50.05%); 正丁醇萃取相中含有5种,主成分为丙基-2-甲基丁酸酯(54.57%)。在所有成分中,酯类物质居多,也有少量的烯、酮、醇、苯和烷烃。1-甲基,4-(1-甲基乙基)-1,4环己二烯、2,2-二亚甲基[6-(1,1-二甲基乙基)-4-甲基]苯酚、1-十八烯和二十烷为石油醚和乙酸乙酯的共有成分; 1、2-苯二甲酸单(2 -乙基己基)酯为乙酸乙酯和正丁醇的共有成分。 该研究进一步明确了大叶桉的化学成分,为其在医药、化工和化感方面的应用研究奠定了基础。

Abstract:

With different polarity organic solvent extracted water leaching solution of Eucalyptus robusta leaf and analyzed by gas chromatography-mass spectrometry(GC-MS)in order to make a clear definition of the chemical components. 37 compounds were identified. Among them,the petroleum ether extraction phase contained 20 kinds and main component was oxalic acid,butyl isohexyl ester(37.24%); Ethyl acetate extraction phase contained 16 kinds and main component was phenol,2,2'-methylenebis[6-(1,1-dimethylethyl)]-4-methyl-(50.05%); N-butanol extraction phase contains 5 kinds and main component was butanoic acid,2-methylpropyl ester(54.57%). Of all the ingredients,ester material in the majority,also had a small amount of olefin,ketones,alcohols,benzene and alkane. 1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-,phenol,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-,1-octadecene and eicosane for petroleum ether and ethyl acetate common composition,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester for ethyl acetate and n-butanol common composition. The study further defined the chemical components of E. robusta and builted the foundation for its application in pharmaceutical,chemical and allelopathy.With different polarity organic solvent extracted water leaching solution of Eucalyptus robusta leaf and analyzed by gas chromatography-mass spectrometry(GC-MS)in order to make a clear definition of the chemical components. 37 compounds were identified. Among them,the petroleum ether extraction phase contained 20 kinds and main component was oxalic acid,butyl isohexyl ester(37.24%); Ethyl acetate extraction phase contained 16 kinds and main component was phenol,2,2'-methylenebis[6-(1,1-dimethylethyl)]-4-methyl-(50.05%); N-butanol extraction phase contains 5 kinds and main component was butanoic acid,2-methylpropyl ester(54.57%). Of all the ingredients,ester material in the majority,also had a small amount of olefin,ketones,alcohols,benzene and alkane. 1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-,phenol,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-,1-octadecene and eicosane for petroleum ether and ethyl acetate common composition,1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester for ethyl acetate and n-butanol common composition. The study further defined the chemical components of E. robusta and builted the foundation for its application in pharmaceutical,chemical and allelopathy.