基于低拷贝核基因GBBSI的禺毛茛复合体系统进化研究

Phylogenetic analysis of Ranunculus cantoniensis complex based on low-copy nuclear gene GBBSI

关键词:  禺毛茛  多倍体复合体  系统进化  GBBSI  枢纽基因组

Key words:  Ranunculus cantoniensis  polyploid complex  phylogeny  GBBSI  pivotal genome

摘要:

禺毛茛多倍体复合体及其近缘种系统进化关系复杂,杂交与多倍化现象同时存在。该复合体内高倍性植物的形成及扩散过程仍需进一步研究。首次克隆了毛茛属植物低拷贝核基因颗粒结合型淀粉合成酶I(GBBSI)基因,并利用其构建禺毛茛多倍体复合体及其近缘种的系统进化树和网状进化关系,进而证明其适合于研究毛茛属植物种下系统发育研究。结果表明:匍枝毛茛与多倍体复合体关系密切,参与了该多倍体复合体的起源和进化; 禺毛茛起源于茴茴蒜和卷喙毛茛,扬子毛茛起源于茴茴蒜和匍枝毛茛; 在该类群中茴茴蒜是个关键种,它在多倍体复合体中可能起着枢纽基因组的重要作用。

Abstract:

Evolutionary relationship of Ranunculus cantoniensis polyploid complex and its allied species was complicated,and hybridization and polyploidization occurred simultaneously in this complex. It was necessary to explore the speciation and dispersal processes of high-ploid taxa. First cloned partial sequence of GBBSI gene,then phylogenetic tree and network were constructed using the introns of GBBSI gene,and proved that the introns of GBBSI gene were suited to study phylogenetic relationship of Ranunculus. The results showed that R. repens closely associated with polyploid complex,participating in the origin and evolution of polyploid complex; R. cantoniensis originated from hybridization of R. chinensis and R. silerifolius var. silerfolius,R. sieboldii originated from hybridization of R. chinensis and R. repens,R. repens might originated from hybridization of R. chinensis and R. silerifolius var. dolicathus; R. chinensis was a key species in this complex,which may play an important role as a pivotal genome.