不同外源激素对灰毡毛忍冬“渝蕾1号”愈伤组织生物量生长和绿原酸含量的影响

Effects of different exogenous hormones on chlorogenic acidcontent and the growth of biomass in callus of Loniceramacranthoides ‘Yulei 1'

关键词:  灰毡毛忍冬‘渝蕾1号'  愈伤组织  绿原酸  生物量

Key words:  Lonicera macranthoides ‘Yulei 1'  callus  chlorogenic acid  biomass

摘要:

为探索外源激素对灰毡毛忍冬‘渝蕾1号'愈伤组织生长及其代谢产物绿原酸含量的影响,该研究通过在培养基中添加不同种类和浓度的外源激素,以诱导其愈伤组织的产生及增殖生长,并采用称量法和高效液相色谱法分别检测愈伤组织生物量和绿原酸含量。结果表明:不同外源激素及其组合对愈伤组织生物量和绿原酸含量的积累产生不同的影响; 培养基中添加单一外源激素时,对愈伤组织生物量影响最大的为TDZ激素; 和对照相比,不同浓度的TDZ均使生物量显著提高,其中0.5 mg·L^-1的TDZ使生物量达最大,为0.62 g; 对绿原酸积累影响的结果不太一致,除TDZ激素影响最小以外,0.5 mg·L^-1的NAA使愈伤组织中的绿原酸含量积累最多,为11.18 mg·g^-1; 外源激素组合中,含有TDZ激素的组合生物量偏高,但绿原酸含量偏低,这与单一外源激素的结果一致。MS+0.5 mg·L^-1 TDZ+0.5 mg·L^-1 2,4-D培养基使愈伤组织中绿原酸含量积累较多,为15.53 mg·g^-1,且生物量达最高,为0.65 g,因此该激素组合为该研究最适培养基组合。通过筛选适宜的外源激素及其组合能促进灰毡毛忍冬‘渝蕾1号'愈伤组织生物量的提高和次生代谢产物绿原酸的积累。研究结果对从灰毡毛忍冬‘渝蕾1号'愈伤组织中获得大量的绿原酸提供了重要依据。

Abstract:

Influence of exogenous hormones on the growth of biomass and the generation of chlorogenic acid in callus of Lonicera macranthoides‘Yulei 1' was investigated in this study. Different kinds and concentrations of exogenous hormones were added in culture media to induce the proliferation anddifferentiation of callus. The biomass and amount of chlorogenic acid in callus were analyzed by weight and HPLC. The result showed that the biomass of callus and amount of chlorogenic acid were affected by variation of hormones. TDZ in all hormones had the strongest influence on the biomass of callus when single exogenous hormone was added. It reached the maximum of 0.62 g in 0.5 mg·L^-1 TDZ. On the other hand,TDZ had the least influence on the generation of chlorogenic acid, whereas 0.5 mg·L^-1 of NAA had the highest amount(11.18 mg·g^-1). In the groups of combination of hormones, the ones with TDZ generated abundant biomass but low chlorgenic acid. The group of MS+TDZ 0.5 mg·L^-1 + 2,4-D 0.5 mg·L^-1 which had the highest biomass(0.65 g)and relatively high chlorogenic acid(15.53 mg·g^-1),was the most appropriate culture media. Therefore, this study provides an important basis on obtaining enormous chlorogenic acid from callus of L. macranthoides ‘Yulei 1' through screening the different exogenous hormones and their combinations.