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不同成熟度老鹰茶中酚类化合物含量及抗氧化活性研究 |
李志荣1,2, 邵起菊3, 吴其妹3, 李影3, 李文阳1, 方灿灿1, 肖世基3, 陈荣祥1*
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1. 遵义医科大学生命科学研究院,贵州 遵义 563000;2. 遵义医科大学第三附属医院,贵州 遵义 563000;3. 遵义医科大学药学院,贵州 遵义 563000
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摘要: |
为了研究不同成熟度老鹰茶(老、嫩叶茶)化学成分含量及抗氧化活性的差异,并对其进行辨识及质量评价,该文首先利用LC-MS/MS法测定老鹰茶中15种酚类化合物,然后采用DPPH自由基清除率、ABTS+自由基清除率、铁离子还原能力评价两种茶叶抗氧化能力,最后通过数据统计分析探讨老、嫩叶老鹰茶化学成分、抗氧化活性的差异以及抗氧化活性的物质基础。结果显示,(1)嫩叶茶和老叶茶中9种成分(儿茶素、对香豆酸、异槲皮苷、金丝桃苷、烟花苷、紫云英苷、山奈酚、柚皮素、阿福豆苷)存在显著差异(P<0.05),聚类分析、主成分分析、正交偏最小二乘判别分析结果均可将二者区分。(2)在抗氧化能力上,方差分析结果显示二者在DPPH自由基清除率、ABTS+自由基清除率、铁离子还原能力之间具有显著性差异(P<0.05),嫩叶茶优于老叶茶。(3)偏最小二乘回归分析法提示老鹰茶15种酚类化合物中的异槲皮苷、儿茶素、紫云英苷、绿原酸、金丝桃苷、对香豆酸、山奈酚是其发挥抗氧化效能的主要化学成分。综上,本研究可为老鹰茶的辨识、质量控制及应用提供依据。 |
关键词: 老鹰茶,酚类化合物,LC-MS/MS,抗氧化活性,相关分析 |
DOI:10.11931/guihaia.gxzw202302010 |
分类号: |
基金项目:国家自然科学基金(31560102,81760652);遵义医科大学大学生创新创业项目(ZYDC2021152, ZYDC2022035)[Supported by National Natural Science Foundation of China (31560102,81760652) and College Students |
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Determination of phenolic compounds in Hawk Tea with different maturity and study on their antioxidant activity |
li zhirong1,, Shao qiju,3, Wu qimei3, Li ying3, Li wenyang1, Fang cancan1, Xiao shiji3, Chen rongxiang1*
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1. Institute of Life Sciences, Zunyi Medical University, Zunyi 563000, Guizhou, China; 2. The Third Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, China; 3. School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China
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Abstract: |
In order to studay the differences in chemical components and antioxidant activities, the identification and quality evaluation of Hawk tea with different maturity. A total of 15 phenolic compounds in Hawk tea were determined by LC-MS/MS, and their antioxidant activities were evaluated by DPPH radical scavenging ability,ABTS+ radical scavenging ability, and ferric reducing antioxidant power (FRAP). Then, the relationship between antioxidant activity and the compounds were studied through statistical analysis. The results were as follows: (1) There were significant differences in 9 active components (catechin, p-coumaric acid, isoquercitrin, hyperoside, nicotiflorin astragalin, kaempferol, naringetol, afzelin ) between the tender leaf and the mature leaf (P<0.05). The results of hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) could well distinguish the two kinds of Hawk tea. (2)The variance analysis of the antioxidant activities showed that there were significant differences in DPPH radical scavenging ability, ABTS+ radical scavenging ability and FRAP between the two kinds of Hawk tea. (3) The relationship between the 15 compounds and antioxidant activity were analyzed by partial least square regression (PLSR) analysis. Results indicated that the compounds have the greatest contribution to the antioxidant activities were isoquercitrin, catechin, astragaline, chlorogenic acid, hyperoside, p-coumaric acid, and kaempferol. This study can provide a basis for the identification, quality control and application of Hawk tea. |
Key words: Hawk tea, phenolic compound, LC-MS/MS, antioxidant activity, correlation analysis |