宽叶金粟兰的化学成分及其抗肿瘤活性研究

Chemical constituents from Chloranthus henryi and their antitumor activities in vitro

关键词:  宽叶金粟兰, 化学成分, 分离纯化, 结构鉴定, 细胞毒活性, 抑制作用

Key words:  Chloranthus henryi, chemical constituents, isolation and purification, structural identification, cytotoxic activity, inhibitory effects

摘要:

为探寻宽叶金粟兰(Chloranthus henryi)的化学成分及抗肿瘤活性,该研究采用硅胶柱层析、反相柱色谱、Sephadex LH-20凝胶和半制备高效液相等色谱技术对宽叶金粟兰95%乙醇提取物的乙酸乙酯部位进行分离纯化,根据理化性质与波谱数据并结合 参考文献鉴定所得化合物的结构,并采用MTT法评价化合物细胞毒活性。结果表明:(1)从该植物的95%乙醇提取物的乙酸乙酯部位分离并鉴定了12个化合物,分别为pipercyclobutanamide C(1)、chololactone A(2)、sarcanolide B(3)、环银线草醇A(4)、chloramultiol D(5)、chlorasessilifol B(6)、chlorajaponol(7)、tianmushanol(8)、spicachlorantins B(9)、spicachlorantins A(10)、及已灵素A(11)、chloramultiols A(12)。化合物1为新化合物,除化合物8外,其余化合物均为首次从宽叶金粟兰中分离得到。(2)细胞毒活性测试结果显示,仅化合物2和化合物7对人宫颈癌细胞HeLa有抑制作用,IC₅₀值分别为(4.50±0.27)μmol·L^-1和(4.25±0.08)μmol·L^-1,其他化合物对其均无抑制作用。该研究结果为进一步深度研究与开发利用金粟兰属草本植物提供了一定的科学依据。

Abstract:

In order to study the chemical constituents from Chloranthus henryi and their antitumor activities in vitro. The chemical constituents of ethyl acetate fraction, extracted with 95% ethanol from C. henryi were isolated and purified by silica gel column chromatography, reversed phase column chromatography, Sephadex LH-20 column chromatography and preparation liquid chromatography, and their structures were identified by the physicochemical properties, spectral data combined with relevant literatures. The cytotoxic activities of these compounds were evaluated by MTT method. The results were as follows:(1)Twelve compounds were isolated and identified as pipercyclobutanamide C(1), chololactone A(2), sarcanolide B(3), oxacol A(4), chloramultiol D(5), chlorasessilifol B(6), chlorajaponol(7), tianmushanol(8), spicachlorantins B(9), spicachlorantins A(10), serrachlorin A(11), chloramultiols A(12). Among them, Compound 1 was identified as a new compound, and except for Compound 8, all the other compounds were isolated from the C. henryi for the first time.(2)The cytotoxic activity test results showed that only compounds 2 and 7 had good inhibitory effects on HeLa cells in vitro, while the other compounds showed no inhibition effects. The IC₅₀ of the two compounds were(4.50±0.27)μmol·L^-1 and(4.25±0.08)μmol·L^-1 respectively. In conclusion, the study enriches the chemical constituents of C. henryi, and provides a reference for the further exploration and utilization of this Chloranthus herb plants.