菌剂施用对高温胁迫下芒萁光合与抗逆生理特性的影响

Effects of bacterial agent application on physiological characteristics of photosynthesis and stress resistance in Dicranopteris pedata under high temperature stress

关键词:  芒萁, 高温胁迫, 纳豆芽孢杆菌, 光合特性, 抗逆生理

Key words:  Dicranopteris pedata, high temperature stress, Bacillus natto, photosynthetic characteristics, stress resistance physiology

摘要:

为研究纳豆芽孢杆菌菌剂对芒萁耐热能力的影响,该研究测定了不同温度和不同肥料处理下当年生芒萁的光合和抗高温生理指标。结果表明:(1)温度和肥料都能显著影响芒萁的光合和抗高温生理(P<0.05)。(2)温度升至45 ℃时,芒萁叶片的净光合速率、蒸腾速率、气孔导度和叶绿素含量均显著降低(P<0.05),高温胁迫对芒萁光合生理的抑制属于非气孔限制; 为抵御高温胁迫,芒萁的超氧化物歧化酶、过氧化物酶活性、脯氨酸、丙二醛含量和相对电导率显著升高(P<0.05)。(3)与对照组和有机肥组相比,菌剂显著提高了芒萁叶片的净光合速率、蒸腾速率、气孔导度、叶绿素含量以及过氧化物歧化酶和过氧化氢酶活性,降低了胞间CO₂浓度、丙二醛含量和相对电导率(P<0.05)。(4)使用主成分分析与隶属函数法相结合的评价方法综合评价了芒萁抗高温能力,发现菌剂添加的有机肥施用下芒萁的抗高温能力得分更高。综上认为,芒萁具有一定的抗高温性,能根据不同胁迫环境选择性地诱导抗逆生理活动来适应高温环境; 纳豆芽孢杆菌菌剂可缓解高温胁迫对芒萁光合作用的抑制,诱导抗氧化酶活性的提高以缓解细胞的损伤,减轻渗透调节的压力,有效提高芒萁对高温胁迫的抵抗能力。该研究结果对南方红壤侵蚀区及类似区域的生态修复和水土保持具有一定意义。

Abstract:

In order to investigate study the influence of microbial agent Bacillus natto on the ability of Dicranopteris pedata to tolerate high temperature stress, we determined the physiological indexes of photosynthesis and resistance to high temperature of annual D. pedata under different temperatures and fertilizer treatments. The results were as follows:(1)Both temperature and fertilizer could significantly affect the photosynthesis and high temperature resistance physiology of D. pedata(P<0.05).(2)The net photosynthetic rate, transpiration rate, stomatal conductance, and chlorophyll content of D. pedata leaves were significantly reduced(P<0.05)when the temperature was increased to 45 ℃. The inhibition of photosynthetic physiology of Dicranopteris pedata by high-temperature stress was an non-stomatal limiting. To defend the high temperature stress, the superoxide dismutase, peroxidase activity, proline, malondialdehyde content, and relative conductivity of D. pedata were increased significantly(P<0.05).(3)Compared with the control group and the organic fertilizer group, the fertilizer with microbial agent audition significantly increased the net photosynthetic rate, transpiration rate, stomatal conductance, chlorophyll content, and the activities of peroxide dismutase and catalase in leaves of D. pedata, and decreased the intercellular CO₂ concentration, malondialdehyde content and the relative electrical conductivity of D. pedata leaves(P<0.05).(4)The evaluation method combining principal component analysis and membership function method was used to evaluate the high temperature resistance of D. pedata. It was found that the D. pedata scored higher in resistance to high temperature under fertilizer-added origin manure application. Overall, D. pedata has a certain degree of resistance to high temperature, and can selectively induce stress resistance physiology to adapt the high temperatures environment according to different stress environments. Bacillus natto can alleviate the photosynthesis inhibition of Dicranopteris pedata by high temperature stress, induce the increase of antioxidant enzyme activities to alleviate cell damage, reduce the pressure of osmoregulation, and effectively improved the resistance of D. pedata induced by high temperature stress. This study provide a reference for the ecological restoration and soil and water conservation in the southern red soil erosion area and similar areas.