垂穗披碱草TCP转录因子家族鉴定及激素响应模式分析

Identification of TCP transcription factor familyand analysis of phytohormone response patterns in Elymus nutans

关键词:  垂穗披碱草, TCP转录因子家族, 全长转录组, 外源植物激素, 表达模式

Key words:  Elymus nutans, TCP transcription factor family, full-length transcriptome, exogenous phytohormones, expression patterns

摘要:

垂穗披碱草(Elymus nutans)是青藏高原地区的优质牧草,具有较高的生态价值和经济价值。TCP是植物特有的转录因子家族,参与调控植物叶片发育、侧枝形成、植物激素合成与信号转导等生理过程。为鉴定垂穗披碱草TCP转录因子家族成员,该研究利用单分子实时(SMRT)测序技术获得了垂穗披碱草全长转录组数据,并对4种激素处理后的垂穗披碱草叶片进行Illumina测序,以探究EnTCPs基因在不同激素处理下的表达模式。结果表明:(1)垂穗披碱草全长转录组测序共获得90 956条非冗余转录本。(2)基于全长转录组数据共鉴定到26个EnTCPs,氨基酸数为186～575 aa,亚细胞定位预测均位于细胞核。(3)根据进化树的分支情况可将26个EnTCPs分为Class I,Class Ⅱ-a(CIN)和Class Ⅱ-b(CYC/TB1)3个亚家族,保守结构域分析表明它们均含有TCP结构域。(4)不同激素处理后的表达模式分析显示,7个EnTCPs家族成员(En108950、En35573、En10347、En16325、En128790、En10346和En14028)在激素处理后上调/下调表达,它们可能参与了激素合成与信号转导通路; qRT-PCR分析结果显示,En35573和En14028参与生长素响应,En108950、En10347、En128790、En10346和En14028均参与细胞分裂素响应,En14028参与脱落酸响应,En108950参与茉莉酸响应,而En16325可能参与多种激素信号转导通路。该研究结果为后续EnTCPs基因功能研究奠定了基础,为研究EnTCPs对激素响应的分子机制提供了参考依据。

Abstract:

Elymus nutans, a high-quality forage distributed on the Qinghai-Tibetan Plateau, holds high ecological and economic value. TCP transcription factors, a plant-specific transcription factor family, play a crucial regulatory role in plant growth and development processes, including leaf development, lateral branch morphogenesis, and phytohormonesynthesis and signal transduction pathways. To identify the TCP transcription factor family members of E. nutans, single molecule real-time(SMRT)sequencing technology was used to obtain full-length transcriptome data from E. nutans. Meanwhile, the Illumina sequencing platform was employed to investigate the response patterns of E. nutans under four phytohormone treatments. The results were as follows:(1)A total of 90 956 non-redundant full-length non-chimeric(FLNC)transcripts were obtained.(2)A total of 26 EnTCPs were identified based on the full-length transcriptome data. Bioinformatics analysis revealed a diverse range of amino acids(186 to 575 aa), with all EnTCPs predicted to be located in the nucleus.(3)Based on the branching of the phylogenetic tree, the 26 EnTCPs were divided into Class I subfamily, Class Ⅱ-a(CIN)subfamily, and Class Ⅱ-b(CYC/TB1)subfamily. Conserved motifs analysis revealed that all EnTCPs possessed a TCP domain.(4)Expression pattern analysis suggested that the expression of 7 EnTCPs family members(En108950, En35573, En10347, En16325, En128790, En10346, and En14028)was up-regulated/down-regulated under different phytohormone treatments, suggesting their potential involvement in hormone synthesis pathways. qRT-PCR analysis results showed that En35573 and En14028 were involved in auxin response, while En108950, En10347, En128790, En10346, and En14028 were all involved in cytokinin response. En14028 was involved in abscisic acid response, En108950 was involved in jasmonic acid response, and En16325 might be involved in multiple hormone signaling pathways. These findings provide a foundation for further exploration of the function of the EnTCPs gene and can serve as a reference.