延龄草化学成分及抗乳腺癌活性筛选研究

Chemical constituents and their anti-breast cancer activities of Trillium tschonoskii

关键词:  延龄草, 化学成分, 分离鉴定, 乳腺癌, MDA-MB-231细胞

Key words:  Trillium tschonoskii, chemical constituent, isolation and identification, breast cancer, MDA-MB-231 cells

摘要:

为探讨延龄草化学成分及其对乳腺癌细胞的影响,该研究采用硅胶和Sephadex LH-20对延龄草提取物进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构,并采用MTT法评价化合物对乳腺癌细胞株MDA-MB-231的抑制活性。结果表明:(1)从延龄草中鉴定出24个化合物,分别为lysicamine(1)、hydroxyframoside(2)、goniothalamusin(3)、pyrocatechol(4)、6-羟基-7,8-二甲氧基香豆素(5)、norisoboldine(6)、4,6-二羟基-2-甲氧基苯乙酮(7)、hinokiresinol(8)、sedanolide(9)、rostratamine(10)、cinnamicacid(11)、magnolioside(12)、platypterophathalide(13)、transtorine(14)、capillasterolide(15)、globularin(16)、芒柄花素(17)、对羟基肉桂酸乙酯(18)、cirsilineol(19)、地芰普内酯(20)、acetoisovanillone(21)、rubrosterone(22)、didehydroconicol(23)、去氢吐叶醇(24); 所有化合物均首次从延龄草中分离鉴定。(2)化合物1、4、8、9、13、14、16、19、23对MDA-MB-231细胞具有不同程度的抑制作用,其中化合物9对MDA-MB-231细胞的抑制作用与环磷酰胺相当(P>0.05)。该研究结果揭示了延龄草的化学成分,其中化合物9具有潜在的抗乳腺癌活性。

Abstract:

In order to investigate the chemical constituents of Trillium tschonoskii and their effects on breast cancer cells. The extract of T. tschonoskii was isolated and purified by silica gel and Sephadex LH-20 and the structures of the compounds were identified based on physicochemical properties and spectral data. The inhibitory activities of the compounds against the breast cancer cell line MDA-MB-231 were evaluated by the MTT method. The results were as follows:(1)Twenty-four compounds been identified from T. tschonoskii as lysicamine(1), hydroxyframoside(2), goniothalamusin(3), pyrocatechol(4), 6-hydroxy-7, 8-dimethoxycoumarin(5), norisoboldine(6), 4,6-dihydroxy-2-methoxyacetophenone(7), hinokiresinol(8), sedanolide(9), rostratamine(10), cinnamicacid(11), magnolioside(12), platypterophathalide(13), transtorine(14), capillasterolide(15), globularin(16), ononin(17), ethyl p-hydroxycinnamate(18), cirsilineol(19), loliolide(20), acetoisovanillone(21), rubrosterone(22), didehydroconicol(23), dehydrovomifoliol(24); all compounds were isolated and identified from T. tschonoskii for the first time.(2)Compounds 1, 4, 8, 9, 13, 14, 16, 19, 23 had inhibitory effects of varying degrees on MDA-MB-231 cells. Among them, the inhibitory effect of Compound 9 on MDA-MB-231 cells was comparable to that of cyclophosphamide(P>0.05). This study result reveals the chemical constituents of T. tschonoskii, among which Compound 9 has potential anti-breast cancer activity.