引用本文: | 王丹丹, 黄 妍, 周中政, 李婷婷, 吴发明, 姚秋阳.不同浓度硒酸钠对茶树的生长和生理指标的影响[J].广西植物,2021,41(2):183-194.[点击复制] |
WANG Dandan, HUANG Yan, ZHOU Zhongzheng, LI Tingting,
WU Faming, YAO Qiuyang.Effects of selenate at different concentrations on growth and physiological indexes of tea tree[J].Guihaia,2021,41(2):183-194.[点击复制] |
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不同浓度硒酸钠对茶树的生长和生理指标的影响 |
王丹丹, 黄 妍, 周中政, 李婷婷, 吴发明, 姚秋阳*
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遵义医科大学 药学院生药学教研室, 贵州 遵义 563000
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摘要: |
该文以一年生扦插苗为材料,采用水培试验,研究不同浓度的硒酸钠(0、0.15、0.3、1.5、3、5、8 mg·L-1)对茶苗的硒积累、植株生长、生理指标和根尖显微结构等参数的影响。结果表明:茶苗的根和新梢中的硒含量与营养液中的硒浓度正相关; 随着硒浓度升高,茶苗的鲜重、侧根数量、根系生物量、光合色素含量、根系活力等生长指标,茶多酚、可溶性蛋白、可溶性糖等茶叶质量指标均呈现先升后降趋势; 而丙二醛、过氧化氢、脯氨酸含量等抗逆生理指标则呈现先降后升趋势。显微结构分析显示在不同硒浓度处理条件下根尖的显微构造存在差异。低硒浓度(0.15、0.3、1.5 mg·L-1)处理的茶苗根尖的皮层薄壁细胞饱满、完好,表皮细胞较小; 高硒浓度(Se≥3 mg·L-1)处理的茶苗根尖的皮层薄壁细胞变形或受损,表皮细胞增厚,表现出胁迫反应。上述结果说明硒对茶树具有双重效应,合适浓度(0.3 mg·L-1)硒对茶树生长和茶叶品质有益,表现为光合作用和根系活力增强,过氧化物和脯氨酸含量降低,生物量增加,茶叶茶多酚含量增加; 硒浓度过高(≥3 mg·L-1)对茶苗的生长和茶叶品质有害,表现为茶苗出现胁迫反应,茶多酚降低。该研究结果为进一步研究硒对茶树的双重作用机制和富硒茶的栽培提供参考。 |
关键词: 富硒茶, 茶多酚, 根系活力, 显微结构, 双重效应 |
DOI:10.11931/guihaia.gxzw202004001 |
分类号:Q945 |
文章编号:1000-3142(2021)02-0183-12 |
基金项目:国家自然科学基金(31760063); 贵州省科技厅社发攻关项目(黔科合支撑 [2016]2854)[ Supported by the National Natural Science Foundation of China(31760063); Key Program of Social Development by Department of Science and Technology of Guizhou Province([2016]2854)]。 |
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Effects of selenate at different concentrations on growth and physiological indexes of tea tree |
WANG Dandan, HUANG Yan, ZHOU Zhongzheng, LI Tingting,
WU Faming, YAO Qiuyang*
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Pharmacognosy Department, School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China
Pharmacognosy Department, School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China
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Abstract: |
Using hydroponic experiments with one-year-old cutting seedlings, we investigated the effects of sodium selenate(Se concentration of 0, 0.15, 0.3, 1.5, 3, 5 and 8 mg·L-1)on selenium accumulation, plant growth, physiological index, and microscopic structure of tea tree. The results were as follows: The selenium contents in the roots and shoot of tea seedlings were positively correlated with the selenium level in the nutrient solution; With the increase of selenium level, the plant growth indexes including the fresh weight of tea seedling, the number of lateral roots, the root biomass, the contents of photosynthetic pigments, and root activity tended to increase initially and then decrease, and the tea quality indexes such as tea polyphenols, soluble protein and soluble sugar contents also presented the similar trend; However, the contents of malondialdehyde, hydrogen peroxide, and proline decreased in treatments of low selenium levels, and then increased in treatments of high selenium levels. Anatomical analysis of the root tips revealed different effects on the microscopic structure of roots under low and high selenium concentration. The cortex parenchyma cells of root treated with the low Se concentration(0.15, 0.3 and 1.5 mg·L-1)were intact and well-shaped, and the root epidermal cells were smaller; Treatments at high selenium level(Se≥3 mg·L-1)demonstrated larger root epidermal cells and damaged parenchyma cells with irregular shape, which showed stress responses. All together, the findings revealed that Se(IV)induced dual effects on tea seedlings. Se-supplementation with appropriate level(0.3 mg·L-1)showed beneficial effects on plant growth and tea quality by improving root activity and photosynthetic efficiency, minimizing the accumulation of peroxide and proline, and increasing both of the biomass and tea polyphenols contents. While excessive-Se(especially at 8 mg·L-1 Se)showed opposite effects on the parameters mentioned above and led to the reduction of plant growth and tea quality. These data might be helpful to explore the mechanism of dual effects of selenate on the growth of tea tree and provide reference for cultivation of Se-enriched tea. |
Key words: Se-enriched tea, tea polyphenols, root activity, microscopic structure, dual effect |
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