不同光质对白及组培苗生长及光合特性的影响

Effects of different light qualities on growth and photosynthetic characteristics of Bletilla striata seedlings in vitro

关键词:  白及,组培苗,光质,生长,光合特性

Key words:  Bletilla striata, tissue culture seedlings in vitro, light quality, growth, photosynthetic characteristics

摘要:

白及的自然繁殖率极低,组培育苗是其种苗繁殖的主要方式之一。为探索提高白及组培育苗质量及缩短育苗周期的高效人工光环境,该文以紫花白及(Bletillastriata)为试验材料,研究LED光质对白及组培苗的生长和光合特性的影响。结果表明:提高红蓝光组合中的蓝光占比,有利于促进白及组培苗的生长和生物量的积累,而白及的球茎大小与红光的占比呈正相关;在红蓝组光合中增加25%的绿光(2R1B1G),可显著提高白及叶片的叶绿素含量和净光合速率,促进组培苗根系和叶片的生长。综上结果表明,2R1B1G处理下的白及组培苗株高、茎粗、叶绿素含量、净光合速率和根系发育均表现出最佳优势,2R1B1G处理的LED光谱可推荐作为白及组培育苗的光质配方。

Abstract:

Bletilla striata has a very low natural reproduction rate, and tissue culture is one of the main ways of its seedling reproduction. In order to explore an efficient artificial light environment for improving the quality of B. striata and shortening its seedling time, the growth and photosynthetic characteristics of B. striata seedlings under different light qualities were studied. The results were as follows: Increasing the proportion of blue light(2R1B1G)in the background of red and blue light was conducive to promoting the growth and biomass accumulation of B. striata, and its bulb size was positively correlated with the proportion of red light; The chlorophyll contents and net photosynthetic rate were significantly increased and the development of roots and leaves were accelerated when adding 25% green light(2R1B1G)in the background of red and blue light. All these results indicate that plant height, bult diameter, chlorophyll concent, P_n and root development are all the best under 2R1B1G treatment. Therefore, the light quality 2R1B1G is recommended as the best light quality recipe for the seedlings of B. striata.