抗风桐的丛生芽诱导与再生

Multiple shoot proliferation and plant regeneration in Pisonia grandis

关键词:  抗风桐, 丛生芽, 增殖, 生根, 移栽

Key words:  Pisonia grandis, multiple shoot, proliferation, rooting, transplanting

摘要:

南海珊瑚岛礁自然植被由于人类干扰和环境变化出现了退化现象,急需进行植被恢复重建。抗风桐作为南海珊瑚岛礁的优势种,在防风固沙以及植被生态恢复等方面发挥着重要作用。该文以抗风桐带腋芽茎段为外植体,研究不同基本培养基、激素对其不定芽增殖和活性炭对生根、移栽的影响,以便建立其种苗快速繁殖和植株再生体系。结果表明:(1)MS基本培养基适合于丛生芽的诱导和增殖,最佳继代培养周期为60 d,最佳的不定芽增殖培养基为MS + 2.0 mg·L^-1 6-BA + 0.1 mg·L^-1 NAA,培养60 d后增殖倍数达5.52;(2)不定芽在MS +1.0 mg·L^-1 IBA培养基中生根率为96.0%,在生根培养基中添加1.6 g·L^-1活性炭后其生根率下降至42.4%;(3)以添加活性炭生根培养获得的组培苗进行移栽成活率高达93.9%,而不添加活性炭生根培养的组培苗移栽成活率仅为78.3%。研究结果可为抗风桐种苗的离体快繁和珊瑚岛礁的植被恢复奠定技术基础。

Abstract:

The natural vegetation of coral islands in the South China Sea has been degraded due to human disturbance and environmental changes, and there is an urgent need for vegetation restoration and reconstruction. Pisonia grandis is a dominant and tool species in the South China coral islands and an important role in windbreak and sand fixation and vegetation restoration. Here the stems of P. grandis were used as explants, the effects of different basal media, plant growth regulators(PGRs)and activated charcoal(AC)on axillary shoot proliferation, rooting and transplanting were studied. A rapid shoot proliferation and plant regeneration system was established. The results were as follows:(1)MS medium was suitable for the shoot proliferation and subculture period was 60 d, and the optimal shoot proliferation medium was MS medium that supplemented with 2.0 mg 瘙 簚 L^-1 6-BA + 0.1 mg 瘙 簚 L^-1 NAA and the shoot proliferation coefficient was 5.52;(2)The rooting percentage was 96.0% on the MS medium supplemented with 1.0 mg·L^-1 IBA, however, the rooting percentage decreased to 42.4% as the medium was supplemented with 1.6 g·L^-1 AC;(3)The plantlets that was cultured on the MS medium supplemented with AC, the transplanting survival percentage was 93.9%, while the transplanting survival percentage was 78.3% on the medium supplemented without AC. This study provides technological basis for large-scale proliferation and regeneration of P. grandis.