小立碗藓LysM型类受体激酶基因家族生物信息学分析

Bioinformatics analysis of lysin motif receptor-like kinase gene family in Physcomitrella patens

关键词:  小立碗藓, LysM型类受体激酶, 基因家族, 生物信息学, 基因结构, 染色体定位, 系统进化关系

Key words:  Physcomitrella patens, lysin motif receptor-like kinase, gene family, bioinformatics, gene structure, chromosome location, phylogenetic relationship

摘要:

植物LysM型类受体激酶(lysin motif receptor-like kinase,LYKs)是植物中发现的一类重要的RLK,在植物生长发育、抵御逆境胁迫等方面具有不可忽视的作用,是植物中基因功能的研究热点。为更好地了解小立碗藓中的LYK基因,该文利用生物信息学的方法对小立碗藓(Physcomitrella patens)LysM型类受体激酶基因家族成员进行鉴定及分析。通过分析小立碗藓LYK家族成员的基本物理信息、基因结构、染色体定位及系统发生关系,初步探讨了其LYK基因结构、进化与功能间的联系。结果表明:(1)小立碗藓中共有21个LYK基因,其氨基酸序列大小在625～755 aa之间,分子量为69.54～82.02 kDa,等电点在5.98～7.78之间。(2)将小立碗藓所有LYK蛋白与3种典型模式植物(水稻、拟南芥和蒺藜苜蓿)的LYK蛋白共同构建系统进化树,所有LYK蛋白被分为4个亚组(LYK-I、LYK-Ⅱ、LYR-I和LYR-Ⅱ)。小立碗藓各亚组内成员的基因结构、保守域特征显示出较为相似的特征,由此推测其可能具有相同或相似的功能。(3)染色体定位发现21个LYK基因集中分布于4条染色体上并出现小型基因簇,这可能与基因功能相联系。该文分析了小立碗藓LysM型类受体蛋白激酶基因家族的基本信息,可为后续深入研究其LYK基因家族成员的生理生化功能奠定基础。

Abstract:

Plant lysin motif receptor-like kinase(LYKs)are an important type of RLK found in plants. They have a non-negligible role in plant growth and development, resistance to adversity stress, etc. It is a gene function in plants research hotspots. In order to better understand the LYK gene in Physcomitrella patens, we identified and analyzed members of the LysM-type receptor kinase gene family of P. patens, using bioinformatics and preliminarily discussed the relationship between LYK gene structure, evolution and function by analyzing the basic physical information, gene structure, chromosomal location and phylogenetic relationship of the LYK family members. The results were as follows:(1)There were 21 LYK genes in the moss, the amino acid sequence size ranged from 625 to 755 aa, the molecular weight ranges from 69.54 to 82.02 kDa, and the isoelectric point ranged from 5.98 to 7.78.(2)A phylogenetic tree was constructed by combining all the LYK proteins of P. patens with the LYK proteins of three typical model plants(Rice, Arabidopsis, and Medicago truncatula). All LYK proteins were divided into four subgroups(LYK-I, LYK- Ⅱ, LYR-I and LYR-Ⅱ). The genetic structure and conserved domain characteristics of the members in each subgroup of P. patens showed relatively similar characteristics, and it was speculated that they might have the same or similar functions.(3)Chromosome mapping found that 21 LYK genes were concentrated on four chromosomes and there were small gene clusters, which may also be related to gene function. This article analyzed the basic information of the LysM receptor-like protein kinase gene family of P. patens, which can lay the foundation for the in-depth study of the physiological and biochemical functions of its LYK gene family members.