麻疯树种子二萜类化学成分的研究

林 琰<sup>1</sup>; 王爱琴<sup>2</sup>; 吕华伟<sup>3</sup>; 张宏建<sup>4*</sup>

引用本文:	林琰, 王爱琴, 吕华伟, 张宏建.麻疯树种子二萜类化学成分的研究[J].广西植物,2021,41(7):1090-1096.[点击复制]
	LIN Yan, WANG Aiqin, LÜ Huawei, ZHANG Hongjian.Diterpenes from seeds of Jatropha curcas[J].Guihaia,2021,41(7):1090-1096.[点击复制]

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麻疯树种子二萜类化学成分的研究
林琰¹, 王爱琴², 吕华伟³, 张宏建^4*
1. 浙江省立同德医院药学部, 杭州 310012;2. 杭州市中医院药剂科, 杭州 310007;3. 浙江工业大学药学院, 杭州 310014;4. 浙江省中医药研究院药用资源研究中心, 杭州 310007

摘要:

为深入了解麻疯树种子的化学成分,有必要对其具有生物活性的二萜类成分进行研究。该研究利用正相硅胶、ODS、制备液相等色谱分离方法,从麻疯树种子的乙醇提取物中共分离得到了6个二萜类化合物,并采用荧光偏振技术对化合物进行蛋白激酶C(PKC)抑制活性的测定。结果表明:根据化合物的理化性质、MS和NMR,并参考相关文献,这6个二萜类化合物分别鉴定为3β-acetoxy-12-methoxy-13-methyl-podocarpa-8,11,13-trien-7-one(1)、4-epi-dehydroabietic acid(2)、3β-hydroxy-19-O-acetyl-pimara-8(9),15-dien-7-one(3)、Jatrophodione A(4)、14-O-acetyl-5,6-epoxy-(14E)-jatrogrossidentadion(5)、2-Hydroxy jatrophone(6)。其中,化合物2、3、6均为首次从该植物中分离得到,且化合物2对PKCβ具有一定的抑制作用。

关键词: 麻疯树, 种子, 化学成分, 二萜, PKCβ抑制剂

DOI：10.11931/guihaia.gxzw201910013

分类号:Q946.91

文章编号:1000-3142(2021)07-1090-07

基金项目:浙江省科技计划项目(2016C31001); 浙江省中医药科技计划项目(2016ZQ001); 国家自然科学基金(81603255)[Supported by Zhejiang Science and Technology Program(2016C31001); Zhejiang Traditional Chinese Medicine Science and Technology Project(2016ZQ001); National Natural Science Foundation of China(81603255)]。

Diterpenes from seeds of Jatropha curcas

LIN Yan¹, WANG Aiqin², LÜ Huawei³, ZHANG Hongjian^4*

1. Department of Pharmacy, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China;2. Department of Pharmacy, Hangzhou Hospital of Traditional Chinese Medical, Hangzhou 310007, China;3. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China;4. Center for Medicinal Resources Research, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China

Abstract:

In order to learn more about the chemical composition of Jatropha curcas seeds, the diterpenes from J. curcas seeds and their protein kinase C(PKC)inhibition activities was necessary to study. The compounds were isolated by silica gel, ODS and preparation HPLC. Six diterpenes were isolated from ethanol extraction of J. curcas seeds. The structures were identified as 3β-acetoxy-12-methoxy-13-methyl-podocarpa-8,11,13-trien-7-one(1), 4-epi-dehydroabietic acid(2), 3β-hydroxy-19-O-acetyl-pimara-8(9),15-dien-7-one(3), Jatrophodione A(4), 14-O-acetyl-5,6-epoxy-(14E)-jatrogrossidentadion(5), 2-Hydroxy jatrophone(6)by physicochemical properties, MS, NMR and some reported data. Compounds 2, 3 and 6 were isolated from J. curcas for the first time, and compound 2 showed inhibitory effect on PKCβ.

Key words: Jatropha curcas, seed, chemical constituents, diterpene, PKCβ inhibitor