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| 引用本文: | 汪汉成, 向立刚, 郑 苹, 蔡刘体, 余知和.青枯病与黑胫病混发烟株发病茎秆组织微生物群落结构与多样性[J].广西植物,2022,42(2):228-239.[点击复制] |
| WANG Hancheng, XIANG Ligang, ZHENG Ping, CAI Liuti, YU Zhihe.Microbial community structure and diversity of tobacco stem tissue in the mixture occurences of bacterial wilt and black shank[J].Guihaia,2022,42(2):228-239.[点击复制] |
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| 青枯病与黑胫病混发烟株发病茎秆组织微生物群落结构与多样性 |
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汪汉成1*, 向立刚1,2, 郑 苹1, 蔡刘体1, 余知和2
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1. 贵州省烟草科学研究院, 贵阳 550081;2. 长江大学 生命科学学院, 湖北 荆州 434025
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| 摘要: |
| 为了解青枯病与黑胫病混发烟株茎秆组织的微生物菌群组成,该文采用Illumina Miseq高通量测序技术研究了青枯病与黑胫病混发烟株发病茎秆和健康烟株未发病茎秆组织的真菌、细菌群落结构与多样性。结果表明:(1)发病茎秆组织中真菌群落丰富度与多样性较健康茎秆组织低,细菌群落丰富度与多样性较健康茎秆组织高。(2)健康茎秆组织中的优势真菌属为隐球菌属(Cryptococcus)、链格孢属(Alternaria)和镰刀菌属(Fusarium),三者相对丰度之和>80%。(3)发病茎秆组织中的优势真菌属为隐球菌属、链格孢属、镰刀菌属和unclassified_f_Davidiellaceae。(4)norank_c_Cyanobacteria和劳尔氏菌属(Ralstonia)为发病茎秆组织的优势细菌属。综上所述,青枯病与黑胫病混发能够显著改变烟株茎秆真菌、细菌群落结构与多样性,破坏其微生物群落的稳定。 |
| 关键词: 青枯病, 黑胫病, 微生物多样性, Illumina Miseq高通量测序 |
| DOI：10.11931/guihaia.gxzw202007058 |
| 分类号:Q945.8 |
| 文章编号:1000-3142(2022)02-0228-12 |
| 基金项目:国家自然科学基金(31960550); 中国烟草总公司科技项目(110202101048[LS-08]); 贵州省科技厅优秀青年人才培养计划(黔科合平台人才 [2017]5619); 中国烟草总公司贵州省公司科技项目(201914,2020XM03)[Supported by National Natural Science Foundation of China(31960550); Science and Technology Project of China National Tobacco Corporation(110202101048[LS-08]); Outstanding Young Talent Training Program of Guizhou Provincial Science and Technology Department(QianKehe Platform Talents [2017] 5619); Science and Technology Project of China National Tobacco Corporation Guizhou Provincial Company(201914, 2020XM03)]。 |
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| Microbial community structure and diversity of tobacco stem tissue in the mixture occurences of bacterial wilt and black shank |
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WANG Hancheng1*, XIANG Ligang1,2, ZHENG Ping1, CAI Liuti1, YU Zhihe2
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1. Guizhou Academy of Tobacco Science, Guiyang 550081, China;2. College of Life Science, Yangtze University, Jingzhou 434025, Hubei, China
1. Guizhou Academy of Tobacco Science, Guiyang 550081, China; 2. College of Life Science, Yangtze University, Jingzhou 434025, Hubei, China
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| Abstract: |
| In order to understand the composition of microbial flora in the stem tissue of tobacco plants mixed occurrence of bacterial wilt and black shank disease. Illumina Miseq high-throughput sequencing technology was used to study the structures and diversities of fungal and bacterial communities in diseased and healthy tobacco stems. The results were as follows:(1)The richness and diversity of the fungal community in the diseased stem tissue were lower than those in the healthy stem tissue, and the richness and diversity of the bacterial community in the diseased stem tissue were higher than those in the healthy stem tissue.(2)Cryptococcus, Alternaria and Fusarium were the dominant fungi in the healthy stem tissue, and the sum of the relative abundances of the three genera were more than 80% of the fungal community.(3)Cryptococcus, Alternaria, Fusarium and unclassified_f_Davidiellaceae were the dominant fungi in the diseased stem tissues.(4)norank_c_Cyanobacteria and Ralstonia were the dominant bacteria in diseased stem tissues. The above results indicate that the mixture of bacterial wilt and black shank disease can significantly change the structures and diversities of fungal and bacterial communities, and can also destroy the stability of microbial community in tobacco stem tissue. |
| Key words: bacterial wilt, black shank, microbial diversity, Illumina Miseq high-throughput sequencing |
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