油橄榄果渣抗氧化部位化学成分的研究

陈根振<sup>1; 2</sup>; 王蝴蝶<sup>1; 2</sup>; 裴 栋<sup>2; 3</sup>; 黄新异<sup>2</sup>; 邸多隆<sup>1; 2; 3*</sup>王蝴蝶; 裴 栋; 黄新异; 邸多隆

引用本文:	陈根振, 王蝴蝶, 裴栋, 黄新异, 邸多隆王蝴蝶, 裴栋, 黄新异, 邸多隆.油橄榄果渣抗氧化部位化学成分的研究[J].广西植物,2022,42(9):1498-1506.[点击复制]
	CHEN Genzhen, WANG Hudie, PEI Dong, HUANG Xinyi, DI Duolong.Chemical components of antioxidant extract in Olea europaea pomace[J].Guihaia,2022,42(9):1498-1506.[点击复制]

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油橄榄果渣抗氧化部位化学成分的研究
陈根振^1,2, 王蝴蝶^1,2, 裴栋^2,3, 黄新异², 邸多隆^1,2,3*王蝴蝶, 裴栋, 黄新异, 邸多隆
1. 甘肃中医药大学药学院, 兰州 730000;2. 中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室, 兰州 730000;3. 青岛市资源化学与新材料研究中心, 山东青岛 266000

摘要:

为了开展油橄榄果渣中主要抗氧化活性成分的研究,该文通过1,1-二苯基-2-苦肼基自由基(DPPH·)法测定了油橄榄果渣不同提取部位清除DPPH·的能力,并采用硅胶、ODS、Sephadex LH-20等柱色谱方法对抗氧化活性部位的化合物进行分离,同时运用核磁共振波谱等方法鉴定了化合物的结构。结果表明:(1)油橄榄果渣的乙酸乙酯部位具有显著的抗氧化活性,其清除DPPH·的半数抑制浓度(IC₅₀)为119.11 μg·mL^-1。(2)从该活性部位分离得到17个化合物,分别鉴定为没食子酸(1)、羟基酪醇(2)、原儿茶酸(3)、酪醇(4)、儿茶素(5)、香草酸(6)、咖啡酸(7)、香草醛(8)、丁香酸(9)、木犀草苷(10)、橄榄苦苷(11)、丁香酚(12)、槲皮素(13)、木犀草素(14)、芦丁(15)、山楂酸(16)、齐墩果酸(17)。其中,化合物1、6、7、9、15为首次从油橄榄果渣中分离得到。该研究明确了油橄榄果渣的抗氧化物质基础,为油橄榄果渣进一步的高值化利用提供了科学依据。

关键词: 油橄榄果渣, 乙酸乙酯部位, 抗氧化活性, 化学成分, 结构鉴定

DOI：10.11931/guihaia.gxzw202111065

分类号:Q946

文章编号:1000-3142(2022)09-1498-09

基金项目:国家重点研发计划(2019YFD1002400, 2019YFD1002404); 甘肃省重点研发计划(20YF3FA022)[Supported by National Key Research and Development Program of China(2019YFD1002400, 2019YFD1002404); Key Research and Development Program of Gansu Province(20YF3FA022)]。

Chemical components of antioxidant extract in Olea europaea pomace

CHEN Genzhen^1,2, WANG Hudie^1,2, PEI Dong^2,3, HUANG Xinyi², DI Duolong^1,2,3*

1. College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China;2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3. Qingdao Center of Resource Chemistry and New Materials, Qingdao 266000, Shandong, China 1. College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China; 2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 3. Qingdao Center of Resource Chemistry and New Materials, Qingdao 266000, Shandong, China

Abstract:

The purpose of this study was to investigate the main antioxidant active components in Olea europaea pomace. The antioxidant abilities of different extracts from O. europaea pomace was determined by 1, 1-diphenyl-2-picrylhydrazyl free radical(DPPH·)scavenging method. Furthermore, the antioxidant compounds were extracted with solvents, isolated by various column chromatography(silica gel, ODS and Sephadex LH-20), and identified by nuclear magnetic resonance spectrum(NMR). The results were as follows:(1)The ethyl acetate extracts of O. europaea pomace exhibited potent antioxidant activity, characterized with half-inhibitory concentration(IC₅₀)at 119.11 μg·mL^-1 to scavenge DPPH·.(2)Seventeen compounds, gallic acid(1), hydroxytyrosol(2), protocatechuic acid(3), tyrosol(4), catechin(5), vanillic acid(6), caffeic acid(7), vanillin(8), syringic acid(9), cynaroside(10), oleuropein(11), eugenol(12), quercetin(13), luteolin(14), rutin(15), maslinic acid(16), oleanolic acid(17), were isolated from the antioxidant parts of O. europaea pomace. Among them, compounds 1, 6, 7, 9, and 15 were isolated from O. europaea pomace for the first time. The study identify the material basis of O. europaea pomace against oxidative stress, which provides a reference for its development and high-value application.

Key words: Olea europaea pomace, ethyl acetate extract, antioxidant activity, chemical components, structural identification