纤枝金丝桃化学成分研究

Chemical components of Hypericum lagarocladum

关键词:  金丝桃属, 纤枝金丝桃, 化学成分, 多环多异戊烯基间苯三酚, 细胞毒活性

Key words:  Hypericum, Hypericum lagarocladum, chemical components, PPAPs, cytotoxicity

摘要:

为了探究滇产植物纤枝金丝桃的物质基础,寻找活性化合物,该文用80%乙醇对纤枝金丝桃地上部分浸渍提取,应用HP-20大孔吸附树脂、硅胶、葡聚糖凝胶、半制备高效液相等色谱技术对纤枝金丝桃的化学成分进行分离纯化,根据波谱数据鉴定化合物的结构。结果表明:(1)从纤枝金丝桃中分离得到15个化合物,分别鉴定为attenuatumione G(1)、uralodin B(2)、chipericumin C(3)、2,5-二羟基-1-甲氧基氧杂蒽酮(4)、1,7-二羟基氧杂蒽酮(5)、1,7-二羟基-4-甲氧基氧杂蒽酮(6)、槲皮苷(7)、芹菜素-7-O-β-D-葡萄糖苷(8)、芹菜素-7-O-β-D-(6″-O-乙酰基)-葡萄糖苷(9)、木犀草素(10)、槲皮素(11)、白桦脂酸(12)、白桦脂酸甲酯(13)、白桦脂酮酸(14)、β-谷甾醇(15)。化合物1-14为首次从该植物中分离得到。(2)采用MTT法对化合物1-14进行体外抗乳腺癌活性测试,结果仅显示化合物3、6、13对2种乳腺癌细胞株MCF-7和MDA-MB-231有一定的抑制作用,其IC₅₀值为48.6～123.5 μg·mL^-1。该研究结果对综合开发利用纤枝金丝桃资源具有理论和应用意义。

Abstract:

The purpose of this paper was to study the material basis and to find the bioactive chemical components of Hypericum lagarocladum. The aerial part of this species was extracted with 80% ethanol, and then the crude extract was isolated and purified by HP-20 macroporous adsorption resin column chromatography(CC), silica gel CC, Sephadex LH-20 CC and semi-preparative HPLC. The structures of isolated compounds were deduced by the spectroscopic data, as well as comparison with the previous literature data. The results were as follows:(1)Fifteen compounds were isolated from H. lagarocladum, and those compounds were identified as attenuatumione G(1), uralodin B(2), chipericumin C(3), 2,5-dihydroxy-1-methoxyxanthone(4),1,7-dihydroxyxanthone(5),1,7-dihydroxy-4-methoxyxanthone(6), quercitrin(7), apigenin-7-O-β-D-glucopyranoside(8), apigenin-7-O-β-D-(6″-O-acetyl)-glucopyranoside(9), luteolin(10), quercetin(11), betulinic acid(12), betulinic acid methyl ester(13), betulonic acid(14), and β-sitosterol(15). Compounds 1-14 are isolated from H. lagarocladum for the first time.(2)In vitro cytotoxicites of compounds 1-14 were evaluated using MTT method against MCF-7 and MDA-MB-231 cell lines. And only compounds 3, 6 and 13 showed weak cytotoxicites with IC₅₀ values ranging from 48.6 to 123.5 μg·mL^-1. These research results provide scientific theoretical and applied implications of H. lagarocladum for its comprehensive development and utilization.