黔产大青化学成分及其抗菌活性研究

Chemical constituents and antibacterial activities of Clerodendrum cyrtophyllum in Guizhou

关键词:  大青, 化学成分, 分离, 鉴定, 抗菌活性

Key words:  Clerodendrum cyrtophyllum, chemical constituents, isolation, identification, antibacterial activities

摘要:

大青(Clerodendrum cyrtophyllum)是苗族地区常用的药用植物。为研究黔产大青的化学成分及其抗菌活性,该研究采用大孔吸附树脂、反相柱色谱、硅胶柱色谱、Sephadex LH-20 柱色谱、半制备高效液相色谱等方法对大青95%乙醇提取物进行分离和纯化,并根据波谱数据鉴定所得化合物结构,采用MTT法评价其抗菌活性。结果表明:从大青中分离得到18个化合物,分别鉴定为木栓酮(1)、α-amyrin palmitate(2)、sambuculin A(3)、22-dehydroclerosterol(4)、isopetasin(5)、taraxasteryl palmitate(6)、豆甾醇(7)、22-dehydroclerosterol-3-O-β-D-(6'-O-margaroyl)-glucopyranoside(8)、3-O-β-D-galactopyranosyl-(24β)-ethylcholesta-5,22,25-trien(9)、肉苁蓉苷D(10)、tortoside F(11)、balanophonin(12)、木犀草苷(13)、类叶升麻苷(14)、木犀草素(15)、acantrifoside E(16)、trichotomside B(17)、肉苁蓉苷C(18),其中化合物2、3、5、6、8-13、16-18均为首次从大青中分离得到。活性测试结果表明,化合物13对乳房链球菌有一定的抑制作用。该研究丰富了大青化学成分,为其在抗奶牛乳房炎药物的研发提供了参考。

Abstract:

Clerodendrum cyrtophyllum is a common medicinal plant used by Miao people. In order to study the chemical constituents and antibacterial activities of C. cyrtophyllum in Guizhou, China. Macroporous adsorption resin, reversed phase column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatagraphy, and semi-preparative high performance liquid chromatography were used to separate and purify 95% ethanol extracts from C. Cyrtophyllum. The antibacterial activities were evaluated by MTT method. The results showed 18 compounds were isolated and identified as friedelin(1), α-amyrin palmitate(2), sambuculin A(3), 22-dehydroclerosterol(4), isopetasin(5), taraxasteryl palmitate(6), stigmasterol(7), 22-dehydroclerosterol-3-O-β-D-(6'-O-margaroyl)-glucopyranoside(8), 3-O-β-D-galactopyranosyl-(24β)-ethylcholesta-5,22,25-trien(9), cistanoside D(10), tortoside F(11), balanophonin(12), luteoloside(13), acteoside(14), luteolin(15), acantrifoside E(16), trichotomside B(17), cistanoside C(18). Among of them, compounds 2, 3, 5, 6, 8-13, 16-18 were all isolated from C. cyrtophyllum for the first time. Besides,biological activity test showed Compound 13 had antibacterial activity against Streptococcus uberis. The study enriches the chemical composition of C. cyrtophyllum, and provides reference for the drug development of anti-mastitis of dairy cows.