络石藤与地瓜藤抑制NO活性及差异性化学成分研究

Inhibition of NO activity and differential chemical constituents of the medicinal parts in Trachelospermum jasminoides and Ficus tikoua

关键词:  络石藤, 地瓜藤, NO抑制, UPLC-Q-TOF-MS/MS, 差异性成分

Key words:  Trachelospermum jaminoides, Ficus tikoua, NO inhibition, UPLC-Q-TOF-MS/MS, differential chemical constituents

摘要:

络石藤为药典记载的常用中药,而地瓜藤在民间部分地区用作络石藤。为明确两种药材抗炎活性及相关化学成分间的差异,初步探讨其可能的药效物质基础,该研究通过检测不同浓度络石藤与地瓜藤醇提物对RAW264.7细胞存活率的影响,采用Griess试剂法测定LPS诱导的RAW264.7细胞上清液NO释放量,利用UPLC-Q-TOF-MS/MS技术对络石藤与地瓜藤醇提物中的化学成分进行分析,并结合多元统计学中的OPLS-DA模型鉴定两种药材的差异性化学成分。结果表明:(1)两种药材皆具有一定的NO抑制作用,络石藤粗提物的抑制作用稍强于地瓜藤。(2)络石藤与地瓜藤的化学成分存在明显差异,络石藤中的21个差异性成分主要为木脂素类,而地瓜藤中的10个差异性成分主要为黄酮类。综上认为,差异性化学成分可能是造成这两种药材抗炎活性差异的主要活性物质。该研究结果为络石藤与地瓜藤的抗炎药效物质基础和质量控制提供了理论依据。

Abstract:

Trachelospermum jaminoides, a liana of family Apocynaceae, is used as classic traditional Chinese medicine recorded in the Chinese Pharmacopoeia, and Ficus tikoua, a liana of family Moraceae, is used as its customary supply in some ethnic districts, both of which are used as remedies for the inflammation-related disease. In order to determine the differences of anti-inflammatory activity and related chemical constituents between the two medicinal materials, the cell viability test was used to detect the cytotoxicity(RAW264.7)of the medicinal parts in T. jaminoides and F. tikoua at different concentrations, and the Griess method was applied to detect the inhibitory effects of the two species on nitric oxide(NO)production in LPS-induced RAW264.7 cells in vitro. The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)combined with the OPLS-DA model in multivariate statistics were used to identify the differential chemical constituents between the medicinab parts in T. jaminoides and F. tikoua. The results were as follows:(1)The NO production inhibition rate of T. jaminoides is stronger than that of F. tikoua.(2)Twenty-one differential chemical components were identified in the stems with leaves of T. jaminoides, most of which were lignans. Ten differential chemical components in the aerial parts of F. tikoua, most flavonoids, were identified. In summary, the differential chemical constituents may be the main active substances that cause the difference in anti-inflammatory activity between the two medicinal materials. This study provides a theoretical basis for the anti-inflammatory medicinal material basis and quality control of the medicinal parts in T. jaminoides and F. tikoua.