| 引用本文: | 陈庭巧, 董晓晓, 袁 涛, 李清道, 别沛婷.单花、有侧花牡丹品种花芽分化特点及内源激素变化[J].广西植物,2023,43(2):368-378.[点击复制] |
| CHEN Tingqiao, DONG Xiaoxiao, YUAN Tao, LI Qingdao, BIE Peiting.Flower bud differentiation characteristics and endogenous hormone changes of in single and lateral-flowered tree peony(Paeonia Sect. Moutan )cultivars[J].Guihaia,2023,43(2):368-378.[点击复制] |
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| 单花、有侧花牡丹品种花芽分化特点及内源激素变化 |
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陈庭巧1, 董晓晓1, 袁 涛1*, 李清道2, 别沛婷1
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1. 北京林业大学 园林学院, 花卉种质创新与分子育种北京市重点实验室, 国家花卉工程技术研究中心,
城乡生态环境北京实验室,园林环境教育部工程研究中心, 林木花卉遗传育种教育部重点实验室,
北京 100083;2. 洛阳市隋唐城遗址植物园管理处, 河南 洛阳 471000
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| 摘要: |
| 为比较单花牡丹品种‘梨花粉'、有侧花牡丹品种‘姊妹游春'和‘云鄂粉'的花芽分化进程、形态特征差异及花芽分化期内源激素的变化,该研究通过解剖和石蜡切片,观察‘梨花粉'‘姊妹游春'和‘云鄂粉'的花芽分化过程,采用酶联免疫吸附分析法测定不同分化阶段‘梨花粉'和‘姊妹游春'的生长素(IAA)、脱落酸(ABA)、赤霉素(GA3)及玉米素核苷(ZR)的含量。结果表明:(1)‘梨花粉'花芽分化仅6个时期; 有侧花品种‘姊妹游春'和‘云鄂粉'花芽分化为11个时期,顶花原基先分化,侧花原基后分化。(2)叶原基分化期至苞片原基分化期,‘姊妹游春'和‘梨花粉'花芽中ABA、GA3及ZR的含量均升高,IAA含量降低; ‘姊妹游春'侧花原基分化起始阶段,ABA、GA3含量降低,ZR、IAA含量升高。(3)‘梨花粉'ABA/IAA比值在形态分化期时最低。综上认为,有侧花牡丹品种花芽分化比单花牡丹品种早,分化时间长,顶花和侧花部分花器官分化期重叠; 较高水平的ZR、IAA以及较低的ABA、GA3有利于启动侧花原基分化。该研究结果为牡丹长花期育种提供了参考依据。 |
| 关键词: 牡丹, 有侧花品种, 花芽分化, 形态, 内源激素 |
| DOI：10.11931/guihaia.gxzw202111068 |
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| 文章编号:1000-3142(2023)02-0366-11 |
| 基金项目:国家林草局行业标准项目(2018-LY-054); 北京林业大学建设世界一流学科和特色发展引导专项(2019XKJS0324) |
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| Flower bud differentiation characteristics and endogenous hormone changes of in single and lateral-flowered tree peony(Paeonia Sect. Moutan )cultivars |
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CHEN Tingqiao1, DONG Xiaoxiao1, YUAN Tao1*, LI Qingdao2, BIE Peiting1
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1. School of Landscape Architecture, Beijing Forestry University, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation &2.Molecular
Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment,
Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in
Forest Trees and Ornamental Plants of Ministry of Education, Beijing 100083, China;3.2. Luoyang Suitangcheng
History Site Botanical Garden, Luoyang 471000, Henan, China
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| Abstract: |
| The flower bud differentiation process, morphological features and endogenous hormone changes during single-flowered Paeonia suffruticosa cultivar ‘Lihua Fen' and lateral-flowered cultivars ‘Zimei Youchun' and ‘Yun'e Fen' were compared in order to provide a reference for the long florescence breeding of tree peony. The flower bud differentiation process of ‘Lihua Fen', ‘Zimei Youchun' and ‘Yun'e Fen' were observed by anatomy and paraffin sections. The contents of auxin(IAA), abscisic acid(ABA), gibberellin(GA3)and zeatin nucleoside(ZR)in ‘Lihua Fen' and ‘Zimei Youchun' at different differentiation stages were determined by enzyme-linked immunosorbent assay(ELISA). The results were as follows:(1)The flower bud differentiation of ‘Lihua Fen' was only 6 stages and that of ‘Zimei Youchun' and ‘Yun'e Fen' were 11 stages. The apical flower primordium differentiated earlier than the lateral flower primordium.(2)The contents of ABA, GA3 and ZR of ‘Zimei Youchun' and ‘Lihua Fen' increased during the leaf primordium differentiating to the bract primordium, while the content of IAA decreased. As for ‘Zimei Youchun', ABA and GA3 contents decreased at the initial stage of lateral flower primordium, while ZR and IAA increased.(3)The ABA/IAA of ‘Lihua Fen' was the lowest at morphological differentiation stage. The study reveals that the lateral-flowered cultivars have earlier and longer flower bud differentiation compared with the single-flowered cultivar, parts of the floral organ differentiation stage of apical and lateral flowers overlaps. Higher ZR and IAA levels and lower ABA and GA3 levels are beneficial to initiate lateral flower primordium differentiation. |
| Key words: tree peony, lateral-flowered cultivar, flower bud differentiation, morphology, endogenous hormone |
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