山樱花品种间花色差异的代谢组学研究

Metabonomics study on flower color differences of Prunus serrulata cultivars

关键词:  山樱花, 花色, 代谢组, 花青素, 代谢通路

Key words:  Prunus serrulata, flowering color, metabolome, anthocyanins, metabolic pathway

摘要:

山樱花是世界著名的观花类植物,花色是其最重要的观赏特征。为探究影响山樱花品种间花色差异的代谢通路及关键代谢产物变化,该文利用LC-MS/MS技术对白色、绿色和粉色的山樱花品种进行花青素靶向代谢组学比较分析。结果表明:(1)共检测到42种花青素物质,主要包含矮牵牛素、飞燕草素、黄酮类化合物、锦葵色素、芍药花素、矢车菊素、天竺葵素和原花青素8种物质。(2)差异代谢花青素25种,包括11种下调、14种上调,其中有7种花青素在粉色花瓣中显著富集。(3)KEGG通路注释发现差异代谢物在花青素生物合成通路中显著富集,结合聚类结果发现矮牵牛素-3-O-葡萄糖苷是山樱花品种间花色差异产生的关键代谢物。该研究揭示了山樱花花色差异的代谢机理,为后续山樱花花色分子调控机制研究提供了一定的理论依据,也为新品种花色改良和选育提供了一定的科学参考。

Abstract:

Prunus serrulata is a famous ornamental plant worldwide. Flower color is the most prominent trait of cherry. In order to analyze the different pathways and key metabolite change in flower color among P. serrulata cultivars, we conducted a comparative analysis of anthocyanin metabolomics among white, green, and pink P. serrulata cultivars using LC-MS/MS. The results were as follows:(1)A total of 42 anthocyanins were detected, mainly including petunidin, delphinidin, flavonoids, malvidin, peonidin, cyanidin, pelargonidin, and procyanidins.(2)A total of 25 anthocyanins were identified as differential metabolites, including 11 down-regulated and 14 up-regulated ones, in which seven anthocyanins with higher abundance in the pink flower.(3)KEGG pathway annotation showed that the differential metabolites were significantly enriched in the anthocyanin biosynthesis pathway; the results of clustering showed that petunidin-3-O-glucoside was the key metabolite for flower color differences of P. serrulata cultivars. This study provides important insights into the metabolic mechanism of flower color differences in P. serrulata and a reference for novel color cultivars and variety breeding.