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| 超临界CO2流体萃取苦丁茶多糖的工艺优化 |
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韦晓洁1,2*, 银慧慧1,2, 孟 菲1,2, 赵 武1,2, 刘 伟1,2,
姜源明1,2, 覃振华1,2, 孙建华1,2, 陈海兰3
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1. 广西壮族自治区兽医研究所, 南宁 530001;2. 广西兽医生物技术重点实验室,
南宁 530001;3. 广西大学 动物科学技术学院, 南宁 530001
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| 摘要: |
| 为了探讨超临界CO2萃取广西苦丁茶中多糖的工艺条件,该研究采用超临界CO2流体萃取技术分离苦丁茶多糖,利用苯酚-硫酸法对苦丁茶多糖含量进行测定,并考察不同萃取温度(35、40、45、50、55、60 ℃)、萃取压力(20、25、30、35、40、45、50 MPa)、萃取时间(30、60、90、120、150 min)、夹带剂(甲醇、95%甲醇、50%甲醇、无水乙醇、95%乙醇、50%乙醇)以及夹带剂(95%乙醇)用量(2.0、2.5、3、3.5、4.0、4.5、5.0 mL·min-1)对多糖得率的影响,通过设计正交实验方案,对超临界CO2萃取广西苦丁茶多糖的提取工艺进行优化。结果表明:通过单因素和正交实验考察了苦丁茶多糖提取的主要影响因素,得到的最佳萃取工艺条件为萃取温度50 ℃,萃取压力40 MPa,夹带剂流量3.5 mL·min-1,萃取时间150 min; 采用苯酚-硫酸法对苦丁茶多糖含量进行测定。在最优萃取条件下得到的苦丁茶多糖的提取率为7.05%。由此可知,采用超临界CO2流体萃取,具有提取温度低、萃取率高、萃取周期短、低耗以及污染小等优点,适用于苦丁茶多糖的提取。 |
| 关键词: 超临界CO2, 苦丁茶, 多糖, 单因素, 正交实验 |
| DOI：10.11931/guihaia.gxzw201706021 |
| 分类号:Q946.889 |
| 文章编号:1000-3142(2018)05-0590-06 |
| Fund project:中国博士后科学基金(2017M613271XB); 广西畜禽疫苗新技术重点实验室开放基金(15-140-31-B-2)[Supported by the Postdoctoral Science Foundation of China(2017M613271XB); Open Foundation of Guangxi Key Laboratory of Animal Vaccines and New Technology(15-140-31-B-2)]。 |
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| Supercritical CO2 fluid extraction process optimization for polysaccharides from Ilex kudingcha |
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WEI Xiaojie1,2*, YIN Huihui1,2, MENG Fei1,2, ZHAO Wu1,2, LIU Wei1,2,
JIANG Yuanming1,2, QIN Zhenhua1,2, SUN Jianhua1,2, CHEN Hailan3
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1. Guangxi Veterinary Research Institute, Nanning 530001, China;2. Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic,
Nanning 530000, China;3. Institute of Animal Science and Technology of Guangxi University, Nanning 530001, China
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| Abstract: |
| In order to discuss the process conditions for extracting polysaccharides from Ilex kudingcha by supercritical CO2 fluid, the polysaccharides from I. kudingcha were extracted and analyzed by supercritical CO2 fluid extraction. Phenol-sulfuric acid method was applied for the determination of polysaccharides. Effects of different extraction temperatures(35, 40, 45, 50, 55, 60 ℃), extraction pressures(20, 25, 30, 35, 40, 45, 50 MPa), extraction time(30, 60, 90, 120, 150 min), cosolvents(methanol, 95% methanol, 50% methanol, ethanol, 95% ethanol, 50% ethanol)and cosolvent flow rates(95% ethanol)(2.0, 2.5, 3, 3.5, 4.0, 4.5, 5.0 mL·min-1)on the polysaccharides extraction rate were investigated and the process conditions for extracting polysaccharides from I. kudingcha by supercritical CO2 fluid were optimized through orthogonal experiments. Single factor and orthogonal experiments were used to investigate the factors on the yield of polysaccharides. The optimal operation conditions included extraction temperature 50 ℃, extraction pressure 40 MPa, extraction time 150 min, cosolvent flow rate 3.5 mL·min-1. The concentration of polysaccharides was determined by phenol-sulfuric acid method and the extraction rate was 7.05% under the optimal extraction conditions. The results showed that supercritical CO2 fluid extraction technology was favorable for extracting polysaccharides from I. kudingcha with the advantages of low extraction temperature, high extraction rate, short extraction period, the environment-friendly and energy-saving. |
| Key words: supercritical CO2 fluid, Ilex kudingcha, polysaccharides, single factor, orthogonal experiments |
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