| 摘要: |
| 核桃又称胡桃,全身是宝,除核桃仁可以直接食用外,其青皮、叶、枝、花、壳等均可入药,具有重要的经济和药用价值。为了进一步开发和利用核桃属植物资源,该研究以核桃叶为材料,采用Folin-酚法,测定核桃叶75%乙醇提取物及石油醚、乙酸乙酯、正丁醇萃取后的各部分多酚的含量,通过DPPH·和ABTS·自由基清除法评价其抗氧化能力,并分析多酚含量与抗氧化能力的关系。结果表明:核桃叶提取物及其各萃取部分均表现出一定的抗氧化活性,其中乙酸乙酯部分、正丁醇部分的IC50均高于VC,核桃叶75%乙醇粗提物(Ext.)与VC相当,且多酚的含量和抗氧化能力呈现正相关关系。这说明核桃叶的提取物可以作为天然的抗氧化剂应用于食品、医疗、化妆品、保健品等行业。该研究结果为进一步开发利用核桃资源和提高核桃的经济效益提供了一定的理论基础。 |
| 关键词: 核桃叶, 多酚, 含量测定, 抗氧化能力 |
| DOI：10.11931/guihaia.gxzw201703017 |
| 分类号:Q946, R931.5 |
| 文章编号:1000-3142(2018)05-0596-06 |
| Fund project:广西壮族自治区八桂学者专项项目; 广西植物研究所基本业务费项目(桂置业15011); 广西植物功能物质研究与利用重点实验室主任基金(ZRJJ2015-14)[Supported by Bagui Scholar Program of Guangxi; Fundamental Research Fund of Guangxi Institute of Botany(15011 ); Director Fund of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization(ZRJJ2015-14)]。 |
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| Determination of polyphenols content and antioxidant capacity of walnut leaf |
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WEI Huan1,2 , YAN Xiaojie1*, YANG Jianwen2, LI Dianpeng1
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1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi
Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;2. Guilin University of Technology, Guilin 541006, Guangxi, China
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| Abstract: |
| Walnut is priceless and has important economic and medicinal values. Besides walnuts can be directly eaten, the green skin, leaves, branches, flowers, shells and so on can be used as medicines. In order to further develop the use of walnut plant resources, we determined polyphenols contents in 75% ethanol extracts of walnut leaves and petroleum ether, ethyl acetate, n-butanol respectively, evaluated the antioxidant abilities of the extracts, and analyzed the relationship between polyphenols contents and antioxidant capacities. Polyphenols contents were determined by F-C method, and the antioxidant capacities were evaluated by DPPH· and ABTS· radical scavenging. The extracts of walnut leaves and their extracts and fractions showed some antioxidant activities. IC50 of ethyl acetate extract fraction and n-butanol extract fraction were higher than VC, Ext. and VC, and the polyphenols contents and antioxidant capacities showed a positive correlation. The extract of walnut leaves could be used as natural antioxidant in food, drugs, cosmetics, health care and other industries. The results of this experiment can provide some theoretical basis for the further development and utilization of walnut resources. |
| Key words: walnut leaf, polyphenols, content determination, antioxidant capacity |
