| 摘要: |
| 该研究选取广西不同类型的两面针为材料,通过查阅文献对两面针基原植物进行考证,用反相高效液相色谱法(RP-HPLC)对两面针原变种及变种中的5种活性成分进行定量分析。结果表明:(1)毛两面针[Zanthoxylum nitidum(Roxb)DC. f. fastuosumHow ex Huang]早已并入两面针原变种[Z. nitidum(Roxb.)DC. var. nitidum]; 虽然毛两面针与毛叶两面针中文名称相似,但不可认为是同一个变种。(2)RP-HPLC结果显示两面针原变种及变种均不含毛两面针素。(3)5种活性成分在不同类型中存在显著性差异。(4)根据5种活性成分含量数据,对9份两面针样品进行聚类分析,结果划分为4组,与两面针形态划分一致。这不仅理清了“毛叶两面针”与“毛两面针”存在的混淆问题,而且还比较了不同形态类型间的活性成分含量,为两面针质量控制和资源的合理应用开发提供了参考。 |
| 关键词: 两面针, 基原考证, 活性成分, 毛两面针素 |
| DOI：10.11931/guihaia.gxzw201804012 |
| 分类号:Q946 |
| 文章编号:1000-3142(2019)04-0531-09 |
| Fund project:广西科学研究与技术开发重大专项计划项目(桂重科1598005-12); 2014年中医药部门公共卫生服务补助资金中药原料质量监测体系建设项目(财社[2014]76号)[Supported by Guangxi Scientific Research and Technology Development Major Special Program(1598005-12); 2014 Public Health Service Subsidy Fund of Chinese Medicine Department “Construction Program of Chinese Medicine Raw Material Quality Monitoring System”([2014]76)]。 |
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| Botanical origin research and simultaneous determination of five effective components in Zanthoxylum nitidum |
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QIN Yunrui1, JIANG Zhen'ou2, LAI Maoxiang2,
HUANG Yunfeng2*, WANG Xinhong3
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1. Guangxi University of Chinese Medicine, Nanning 530200, China;2. Guangxi Institute of Chinese Medicine &3.Pharmaceutical Science,
Nanning 530022, China, 3. China Resources Sanjiu Medical &4.Pharmaceutical Co., Ltd., Shenzhen 518110, Guangdong, China
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| Abstract: |
| In this study, different phenotypic types of Zanthoxylum nitidum from Guangxi were selected to illustrate the original scientific name of Zanthoxyli Radix through literature review and conduct the quantitative analyses of five effective components based on RP-HPLC. The results were as follows:(1)Zanthoxylum nitidum f. fastuosum that shared the similar Chinese name with Z. nitidum var. tomentosum but was distinctly different between each other had already been incorporated into Z. nitidum var. nitidum;(2)Toddaloactone was not included in both Z. nitidum var. nitidum and Z. nitidum var. tomentosum;(3)The content of effective components in different types was significantly distinct;(4)Clustering analysis based on the content of five effective components separated all populations into four groups, corresponding to phenotypic delimitation. Totally, this study not only clarified the confusion between the Z. nitidum var. tomentosum and Z. nitidum var. nitidum Type 3, but also illustrated the significant difference of effective components in four phenotypes. All results provide guidance for quality control and the rational application and development of resources in Z. nitidum. |
| Key words: Zanthoxylum nitidum, original scientific name, effective components, toddaloactone |
