圆叶牵牛化学成分研究

Chemical constituents of Pharbitis purpurea

关键词:  圆叶牵牛, 化学成分, 三萜, 甾体

Key words:  Pharbitis purpurea, chemical constituents, triterpene, steroid

摘要:

圆叶牵牛植物资源丰富但全草少有研究,对其进行系统的化学成分与生物活性认识很有必要。该文对采自云南大理的圆叶牵牛干燥全草化学成分进行了系统地研究,通过75%乙醇冷浸提取,浸膏以水分散,依次以乙酸乙酯和正丁醇萃取。结果表明:采用硅胶、Sephadex LH-20、RP-18等多种材料柱层析结合重结晶等方法,从圆叶牵牛醇提物乙酸乙酯部分分离得到12个单体化合物,运用现代波谱学技术分别鉴定为木栓酮(1),β-木栓烷醇(2),β-香树脂醇(3),α-香树脂醇(4),6β-羟基豆甾-4-烯-3-酮(5),胡萝卜苷(6),β-谷甾醇(7),豆甾醇(8),7-羟基香豆素(9),对香豆酸-对羟基苯乙醇酯(10),山奈酚香豆酰基葡萄吡喃糖苷(11),单棕榈酸甘油酯(12)。化合物2-5、10-12为首次从该属植物中分离得到。

Abstract:

There were abundant resources of Pharbitis purpurea, but lack of the systematic and deep studies about the whole plant. It is necessary to investigate the chemical compositions and biological activities of P. purpurea. For better understanding the chemical profile, the major constituents of the whole herb of P. purpurea collected from Dali, Yunnan, were systematically studied. After the whole herb of P. purpurea (4 kg) were extracted with 75% EtOH-H₂O solution, the resulting extract was concentrated and suspended in water, and partitioned successively with EtOAc and n-BuOH. Twelve compounds were isolated from EtOAc-soluble part by various column chromatography methods including silica gel, Sephadex LH-20 and RP18 column chromatography, as well as recrystallization method. Based on the spectral analysis, the compounds were identified as friedelin(1), β-friedelinol(2), β-amyrin(3), α-amyrin(4), 6β-hydroxy-stigmast-4-en-3-one(5), daucosterol(6), β-sitosterol(7), stigmasterol(8), umbelliferone(9), p-hydroxyphenylethanol p-coumarate(10), kaempferol-3-β-D-(6-O-cis-p-coumaroyl)glucopyranoside(11), and glyceroylmonopalmitate(12). Compounds 2-5 and 10-12 were isolated from genus Pharbitis for the first time.