| 摘要: |
| 多刺绿绒蒿(Meconopsis horridula )为罂粟科绿绒蒿属一年生草本植物,是一种极具观赏价值和药用价值的高山植物,目前处于濒危状态,因此研究多刺绿绒蒿种子的萌发特性对其种子育苗及人工栽培具有重要意义。为了提高多刺绿绒蒿的种子发芽率,该研究以多刺绿绒蒿的种子为材料,分析了不同消毒剂、浸种时间、温度和外源植物激素对种子萌发特性的影响。结果表明:(1)最适消毒方法为75%乙醇1 min + 3% H2O2 5 min,最适浸种时间为24 h,最适温度和光照条件为20 ℃/10 ℃(光照12 h/黑暗12 h),用无菌水浸种后的种子发芽率为49.67%。(2)GA3 100 ～ 600 mg·L-1和NAA 5 ～ 30 mg·L-1可以提高种子的发芽率、发芽势和发芽指数,缩短发芽启动时间和发芽持续时间,对种子的萌发有促进作用。(3)6-BA 5 mg·L-1和10 mg·L-1对种子的萌发有一定的促进作用,但不显著,6-BA浓度≥15 mg·L-1则抑制种子的萌发。(4)用GA3 500 mg·L-1浸种后的种子发芽指标最好,发芽率、发芽势和发芽指数分别为69.67%、33.00%、4.51,种子的发芽起始时间和发芽持续时间分别为10.67 d、11.67 d。 |
| 关键词: 多刺绿绒蒿, 种子, 萌发, 消毒剂, 浸种时间, 温度, 外源植物激素 |
| DOI：10.11931/guihaia.gxzw201810036 |
| 分类号:Q945.35,R282.2 |
| 文章编号:1000-3142(2019)07-0902-08 |
| Fund project:西藏自治区高校青年教师创新支持计划项目(QCZ2016-54); 第四次全国中药资源普查西藏自治区(中)藏药资源普查项目(20180611-542226)[Supported by the Innovation Support Program for University Young Teachers of Tibet Autonomous Region(QCZ2016-54); Program of the Fourth National Survey of Traditional Chinese Medicine Resources in Tibet Autonomous Region(20180611-542226)]。 |
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| Seed germination characteristics of Tibetan medicine Meconopsis horridula |
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YUAN Fang1,2, SONG Kaijie1, CAI Xitong1, LAN Xiaozhong1,2*
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1. Tibet Agriculture and Animal Husbandry University, Nyingchi 860000, Tibet, China;2. Tibet Agriculture and Animal Husbandry
University-Southwest University, Medicinal Plants Joint Research and Development Centre, Nyingchi 860000, Tibet, China
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| Abstract: |
| Meconopsis horridula is an endangered annual herb belonging to family Papaveraceae. M. horridula is a rare alpine flower, and is a kind of traditional Tibetan medicine. In order to provide a scientific reference for the seed bree-ding and artificial cultivation of M. horridula, the germination characteristics of seeds were studied under the different conditions of disinfector, soaking time, temperature and exogenous plant hormone. The results were as follows:(1)The optimum conditions of seed disinfection method, soaking time, temperature and illumination were 75% ethanol 1 min + 3% H2O2 5 min, 24 h and 20 ℃/10 ℃(illumination 12 h/darkness 12 h), respectively, and the germination rate of disinfected seeds after soaked in sterile water 24 h was 49.67%.(2)Both GA3 100-600 mg·L-1 and NAA 5-30 mg·L-1 had effects on promotion the germination of seeds, mainly by improving seed germination rate, germination potential and germination index, and shortening germination start time and germination duration time.(3)6-BA 5 mg·L-1 and 10 mg·L-1 both could promote the germination of seeds, but the effects were not significant, and when the 6-BA concentration higher than 15 mg·L-1, the germination of seeds were inhibited.(4)The germination data of M. horridula seeds soaked with GA3 500 mg·L-1 were the best. The germination rate, germination energy and germination index of seeds were 69.67%, 33.00% and 4.51, respectively, and the germination start time and germination duration time of seeds were 10.67 d and 11.67 d, respectively. |
| Key words: Meconopsis horridula, seed, germination, disinfector, soaking time, temperature, exogenous plant hormone |
