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| 无瓣海桑果实提取物对衰老小鼠学习记忆能力的影响及其机制研究 |
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易湘茜1,2,3, 李家怡1, 杜正彩1, 高程海1, 郝二伟1,3, 邓家刚1,3*
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1. 广西中医药大学 药学院/海洋药物研究院, 南宁 530020;2. 暨南大学 中药学博士后流动站,
广州 510632;3. 广西药用植物研究所, 中药学博士后工作站, 南宁 530023
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| 摘要: |
| 无瓣海桑果实为真红树无瓣海桑的果实。研究无瓣海桑果实不同提取物对D-半乳糖所致衰老小鼠学习记忆能力影响及其作用机制。采用Morris水迷宫实验测量无瓣海桑果实不同提取物对小鼠的学习记忆能力影响,HE染色观察各组小鼠脑部神经细胞的变化情况,并测定脑组织超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、一氧化氮(NO)含量和单胺氧化酶(MAO)活力。结果表明:与模型组相比,无瓣海桑果实不同提取物处理组小鼠在水迷宫实验中逃避潜伏期明显缩短(P<0.05),目标象限停留时间明显增加(P<0.05)。无瓣海桑果实不同提取物处理组小鼠脑部神经细胞损伤与模型组相比明显减少,小鼠脑部SOD酶活力和GSH-Px酶活力提高(P<0.05),NO含量和MAO活力在脑部显著降低(P<0.05)。无瓣海桑果实不同提取物对D-半乳糖致衰老小鼠学习记忆能力有改善作用,无瓣海桑果实不同提取物通过提高小鼠脑内源抗氧化酶(SOD、GSH-Px)活力,降低脑部NO含量和MAO活力来提高D-半乳糖致衰老小鼠的学习记忆能力。 |
| 关键词: 无瓣海桑果实, D-半乳糖, 学习记忆能力, 神经细胞, 抗氧化活性 |
| DOI：10.11931/guihaia.gxzw201807027 |
| 分类号:Q946, R285.5 |
| 文章编号:1000-3142(2019)11-1534-07 |
| Fund project:广西自然科学基金(2015GXNSFBA139195); 广西中药药效研究重点实验室建设项目(17-259-20); 中国博士后科学基金面上项目(2016M602920XB); 广西中医药大学岐黄工程高层次人才团队培育项目(2018006)[Supported by Natural Science Foundation of Guangxi(2015GXNSFBA139195); Guangxi Key Laboratory on Generic Technology Research and Development of TCM Preparation(17-259-20); Postdoctoral Science Foundation of China(2016M602920XB); Development Program of High-Level Talent Team under Qihuang Program of Guangxi University of Chinese Medicine(2018006)]。 |
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| Effects and mechanisms of Sonneratia apetala fruit extracts on learning and memory abilities in aging mice |
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YI Xiangxi1,2,3, LI Jiayi1, DU Zhengcai1, GAO Chenghai1, HAO Erwei1,3, DENG Jiagang1,3*
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1. School of Pharmaceutical Sciences/Institutes of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530020,
China;2. Post-Doctoral Research Station of Chinese Medicine, Jinan University, Guangzhou 510632,
China;3. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
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| Abstract: |
| To study the effects and mechanism of different extracts of Sonneratia apetala fruit on learning and memory abilities of aging mice induced by D-galactose, the learning and memory abilities of mice were measured by Morris Water Maze Test and the changes of brain nerve cells were observed by HE staining. The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px), nitric oxide(NO)content, and monoamine oxidase(MAO)activity in brain tissue were measured. The results showed that compared with the model group, the escape latency of the mice treated with different extracts from the S. apetala fruit was significantly shorter than that of the model group(P<0.05), and the residence time of the target quadrant was significantly increased(P<0.05)in the water maze experiment. Compared with the model group, the brain nerve cell injury of the mice treated with different extracts was significantly decreased, and the activities of SOD and GSH-Px in the brain of the mice were increased significantly(P<0.05). The content of NO and the activity of MAO in the brain decreased significantly(P<0.05). Different extracts of S. apetala fruits can improve the learning and memory abilities of aging mice induced by D-galactose. Different extracts of S. apetala fruits may reduce the damage of nerve cells in the brain of aging mice and increase the endogenous antioxidant enzymes(SOD, GSH-Px)activity, decreased brain NO content and MAO activity, which can increase learning and memory in aging mice. |
| Key words: Sonneratia apetala fruit, D-galactose, learning and memory, nerve cells, antioxidant capacity |
