摘要: |
金槐是广西特色中药材,具有止血凉血等功效。不同来源的金槐槐米质量具有一定区别,但生产中常将其看做同一类别,严重影响了以槐米为原料的药材质量。该研究以8个广西栽培金槐主流品种(金槐J2、金槐J3、金槐J4、金槐J5、金槐J6、金槐J7、金槐J8、金槐J9)和三种不同年限的野生金槐(20 a以下、20~30 a、30 a以上)槐米为材料,采用傅里叶红外光谱法和紫外分光光度法对其红外光谱特征及芦丁含量进行了测定。结果表明:(1)金槐J3和3个不同生长年限的野生金槐槐米颗粒较大。(2)栽培品种金槐槐米和野生金槐槐米化学成分类似,但化学成分含量存在一定差异。所测槐米样品芦丁含量在22.85%~36.78%之间,其顺序依次为20~30 a野生金槐>30 a以上野生金槐>金槐J3>金槐J6>20 a以下野生金槐>金槐J2>金槐J8>金槐J4>金槐J9>金槐J5>金槐J7,11种样品间存在极显著差异(P<0.01)。(3)基于芦丁含量制定的质量分级标准中,20~30 a野生金槐符合一级标准,金槐J2、金槐J3、金槐J6、20 a以下野生金槐以及30 a以上野生金槐符合二级标准,金槐J4、金槐J5、金槐J7、金槐J8、金槐J9符合三级标准。(4)栽培金槐可作为野生金槐的替代品,金槐J3有效成分含量高、品相好,更适于工业提取芦丁和金槐茶等产品的研发。 |
关键词: 金槐, 品种, 芦丁, 比较 |
DOI:10.11931/guihaia.gxzw201811029 |
分类号:Q946, R284.2 |
文章编号:1000-3142(2019)11-1541-09 |
Fund project:国家自然科学基金(31860169); 农业科技成果转化资金项目(桂科转1222017-13); 广西创新驱动发展专项资金项目(桂科AA17204056); 广西青年自然科学基金(2017GXNSFBA198011); 广西科学院基本科研业务费项目(2017YJJ23010)[Supported by the National Natural Science Foundation of China(31860169); Agricultural Scientific and Technological Achievements Transformation Fund Program(1222017-13); Science and Technology Major Program of Guangxi(AA17204056); Natural Science Foundation of Guangxi(2017GXNSFBA198011); Basic Business Expenses Program of Guangxi Academy of Sciences(2017YJJ23010)]。 |
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Comparison on infrared spectra and rutin content of Sophora japonica ‘Jinhuai' in Guangxi |
WEI Yuanlin1, SHI Yancai2*, ZOU Rong2, TANG Jianmin2, JIANG Yunsheng2,
XIONG Zhongchen2, CHEN Jianxiong3
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1. Beijing University of Chemical Technology, Beijing 102200, China;2. Guangxi Institute of Botany, Guangxi Zhuang
Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;3. Quanzhou County
Dongtai Agricultural Development Co., Ltd., Guilin 541006, Guangxi, China
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Abstract: |
Sophora japonica ‘Jinhuai' is a traditional Chinese medicine from Guangxi, which has the effects of stopping bleeding and cooling blood. Bud quality of Sophora japonica ‘Jinhuai' from different sources is different to some extent, but it is often regarded as the same category in the production, which seriously affects the quality of the medicinal with Sophora japonica ‘Jinhuai' as the raw material. To compare the infrared spectra and rutin content of eight main varieties(Jinhuai J2, Jinhuai J3, Jinhuai J4, Jinhuai J5, Jinhuai J6, Jinhuai J7, Jinhuai J8, Jinhuai J9)and three different seedlings(below 20 a, 20 to 30 a, above 30 a)of Sophora japonica ‘Jinhuai' in Guangxi, fourier transform infrared spectroscopy and ultraviolet spectrophotometry were used to determine its infrared spectral characteristics and rutin content. The results were as follows:(1)Jinhuai J3 and wild seedling with three different growth had larger particles.(2)The chemical composition of Sophora japonica ‘Jinhuai' from different cultivated varieties and seedling was similar, but there were some differences in the content. The rutin content of samples was 22.85% to 36.78%, which was in the order seedling of 20 to 30 a > seedling of above 30 a > Jinhuai J3 > Jinhuai J6 > seedling of below 20 a > Jinhuai J2 > Jinhuai J8 > Jinhuai J4 > Jinhuai J9 > Jinhuai J5 > Jinhuai J7, there were significant differences among the eleven samples(P<0.01).(3)For the quality classification standard based on rutin content, only seedling of 20 to 30 a conforms to the first class standard, while Jinhuai J2, Jinhuai J3, Jinhuai J6, below 20 a and above 30 a of seeding meet the second class standard. Jinhuai J4, Jinhuai J5, Jinhuai J7, Jinhuai J8 and Jinhuai J9 meet the third class standard. Jinhuai J3 had high content of active ingredients and good quality.(4)Variety of Sophora japonica ‘Jihuai' is more suitable for the research and development of products such as industrial extraction rutin and tea, and can be used as the first choice for purchasing or expanding cultivation. |
Key words: Sophora japonica ‘Jinhuai', variety, rutin, compare |