罗汉果多糖对环磷酰胺所致的免疫抑制小鼠免疫功能的影响

Effects of Siraitia grosvenorii polysaccharides on immune function in immunosuppressed mice induced by cyclophosphamide

关键词:  罗汉果, 免疫抑制, 小鼠, 环磷酰胺, 多糖

Key words:  Siraitia grosvenorii, immunodepression, mice, cyclophosphamide, polysaccharides

摘要:

该研究将小鼠随机分成正常组,模型组,罗汉果多糖低、中、高剂量组(25、50、100 mg·kg^-1)和左旋咪唑组,采用腹腔注射环磷酰胺(20 mg·kg^-1)建立免疫抑制小鼠模型,连续灌胃给药14 d后,测定各组小鼠的免疫器官指数、廓清指数(K)、吞噬指数(α)、T和B淋巴细胞增殖水平、耳肿胀度、半数溶血值(HC₅₀)以及免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、IL-2、IL-4、IL-6、TNF-α的含量,并观察脾组织病理形态变化,考察罗汉果多糖对免疫抑制小鼠免疫功能的影响。结果表明:罗汉果多糖各剂量组(25、50、100 mg·kg^-1)均能显著提高免疫抑制小鼠的免疫器官指数、半数溶血值(HC₅₀)、B淋巴细胞增殖能力,明显降低耳肿胀度,显著增加IgG、IgM、IL-2、IL-4、IL-6、TNF-α的含量。罗汉果多糖中、高剂量组(50、100 mg·kg^-1)能显著增强T淋巴细胞增殖能力,明显增加廓清指数(K)、吞噬指数(α)。脾组织病理学观察结果表明,罗汉果多糖可以减轻免疫抑制小鼠脾脏的病理损伤。这表明罗汉果多糖能明显增强环磷酰胺所致免疫抑制小鼠的免疫功能。

Abstract:

In order to study the activity of Siraitia grosvenorii polysaccharides(SGP), specially its immunoregulatory activity, Kunming mice were randomized into six groups, including normal group, model group, SGP groups(25, 50, 100 mg·kg^-1)and levamisole group. Mice were injected with cyclophosphamide(20 mg·kg^-1)to establish the immunosuppressive model, then all groups of mice were treated for sequential 14 d by giving different dose of SGP. Effects of SGP on immune function of immunocompromised mice was studied by detecting many indices, such as thymus and spleen index, carbon clearance index, phagocytic index. Some indexes liked ear edema, the concentration causing 50% hemolysis(HC₅₀), the proliferation ability of T cells and B cells, the contents of IgG, IgM, IL-2, IL-4, IL-6, TNF-α were also be very important parameters to measure immune function. Histopathological examination of spleen tissue also was observed. We found that SGP groups(25, 50, 100 mg·kg^-1)was significantly in improving the thymus and spleen indexes. The concentration causing 50% hemolysis(HC₅₀)was increased effectively. The level activity of serum hemolysin was rising compared to model group. Data also showed that SGP groups(25, 50, 100 mg·kg^-1)could significantly reduce ear edema. Experiments showed that the proliferation ability of B cells, the contents of IgG, IgM, IL-2, IL-4, IL-6,TNF-α were improving. SGP middle and high groups(50, 100 mg·kg^-1)also could significantly increase the carbon clearance index and phagocytic index, and enhance the proliferation ability of T cells. Spleen tissue damage induced by cyclophosphamide was ameliorated according to the histopathological examination. This indicates that SGP can significantly improve immune function of immunocompromised mice.