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甘薯叶片超氧化物歧化酶基因克隆及测序
黄明; 郑学勤; 邵寒霜;   
1.广西师范大学生物系;2.热带作物生物技术国家重点实验室
摘要:
以甘薯(Ipomoeabatatas(L.)Poir)叶为材料提取植物总RNA,经反转录后,利用多聚酶链式反应技术,扩增并克隆超氧化物歧化酶基因的cDNA,并进行测序分析。该序列全长482bp,其读码框编码152个氨基酸,与国外文献报道的甘薯块根SOD基因的cDNA序列相比,具有99%的同源性。
关键词:  超氧化物歧化酶基因  序列  多聚酶链式反应  甘薯  
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