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不同氮源对悬浮培养玫瑰茄细胞的生长及硝态氮同化指征的影响
侯学文; 郭勇;   
华南理工大学生物工程系
摘要:
本文通过改变培养基中的氮源组成,来研究氮源的变化对悬浮培养玫瑰茄细胞的生长及硝态氮同化指征(活体内硝酸还原酶活力)的影响。实验表明,仅在含NO-3的培养基中能检测到硝酸还原酶活力,而仅含NH+4的配方不能检测到该酶活力,表明硝酸还原酶是底物诱导酶。还探讨了培养基中不同的氮源组成对细胞生物量及pH的影响。
关键词:  氮源  悬浮培养  玫瑰茄  硝酸还原酶  
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