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HPLC法测定藜豆中左旋多巴的含量
黄海滨;苏健;谭叶憧
1.广西中医学院制药厂;2.广西中医学院药学院 广西 南宁 530001;3.广西 南宁 530001
摘要:
采用HPLC法建立测定藜豆中左旋多巴含量的方法。色谱柱为岛津C-18甲基硅烷柱(250 min×4.6mm,5μm),流动相为甲醇:0.1 mol/L醋酸溶液(25:75,v/v),以硫唑嘌呤为内标物,检测波长280 nm,流速1.0 mL/min,柱温为室温,供试品的前处理方法为微波辅助提取法。结果表明,该方法准确度高,线性关系好,重现性好,平均加样回收率为97.34%,RSD 1.01%(n=5),结果满意。
关键词:  藜豆    左旋多巴    高效液相色谱法
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