| 摘要: |
| 该文以14个扦插培育的甜叶菊品种叶为材料,从8种不同型号的树脂中筛选出一种合适的大孔吸附树脂对甜叶菊叶中绿原酸类成分进行纯化前处理,采用HPLC法对不同甜叶菊品种叶中所含绿原酸类成分进行比较分析。结果表明:(1)在8种不同型号的树脂中,XAD-16对甜叶菊叶中绿原酸类成分吸附-解析性能最佳。(2)经优化,上样液浓度1.20 mg·mL-1、样品溶液pH 3、解析液乙醇体积分数70%时XAD-16树脂对甜叶菊叶中绿原酸类成分具有较好的纯化效果。(3)HPLC检测分析表明,在14个品种中共检测出新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C六种绿原酸类成分,其中主要成分均为异绿原酸A、绿原酸、异绿原酸C,而在品种3、5、13、14中没有检测出异绿原酸B。(4)14个品种中6个绿原酸类成分的含量分别为异绿原酸 A 20.55～54.3 mg·g-1、绿原酸17.96～32.93 mg·g-1、异绿原酸C 4.15～19.49 mg·g-1、新原酸0.61～4.61 mg·g-1、隐绿原酸0.52～3.11 mg·g-1、异绿原酸B 0.0～3.17 mg·g-1,6种绿原酸类成分总量为43.9～97.8 mg·g-1。可见,不同品种甜叶菊叶中绿原酸类成分含量有明显差异,富含绿原酸类成分的甜叶菊品种可用于开发获取绿原酸类物质。 |
| 关键词: 甜叶菊, 绿原酸类成分, 甜叶菊叶, XAD-16树脂 |
| DOI：10.11931/guihaia.gxzw201901024 |
| 分类号: |
| 文章编号:1000-3142(2020)05-0696-10 |
| Fund project:宁夏大学引进人才科研启动项目(BQC2012001)[Supported by Ningxia University Introduced Talent Research Start-up Program(BQC2012001)]。 |
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| Variation of chlorogenic acid components in leaves of various Stevia rebaudiana cultivars |
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GUO Zhilong, MA Zhihua, ZHANG Hong, CHEN Ren, ZHANG Ziping*
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Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological
Resources in the West, Ningxia University, Yinchuan 750021, China
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| Abstract: |
| The leaves of 14 Stevia rebaudiana cultivars cultured by cuttings were used as experimental materials. Firstly, a suitable macroporous adsorption resin was screened from eight different types of resins to purify chlorogenic acid components in Stevia rebaudiana leaves. Then, the chlorogenic acid components contained in the leaves of different S. rebaudiana cultivars were compared and analyzed by HPLC in order to screen the S. rebaudiana with high chlorogenic acid components. The results were as follows:(1)Among the eight tested resins, XAD-16 showed much better adsorption/desorption capacity for chlorogenic acid components.(2)XAD-16 resin had better purification effect on the chlorogenic acid components in the leaves of S. rebaudiana when the concentration of sample solution was 1.20 mg·g-1 with pH 3 and 70%(v/v)ethanol used as desorption solution.(3)HPLC analysis showed that six kinds of chlorogenic acids were detected in the leaves of 14 S. rebaudiana cultivars, including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C. The main components were isochlorogenic acid A, chlorogenic acid and isochlorogenic acid C, but no isochlorogenic acid B was detected in varieties 3, 5, 13 and 14.(4)The contents of six chlorogenic acids in the leaves of 14 S. rebaudiana cultivars were 20.55-54.3 mg·g-1( isochlorogenic acid A ), 17.96-32.93 mg·g-1( chlorogenic acid ), 4.15-19.49 mg·g-1( isochlorogenic acid C ), 0.61-4.61 mg·g-1( neochlorogenic acid ), 0.52-3.11 mg·g-1( cryptochlorogenic acid ), 0.0-3.17 mg·g-1( isochlorogenic acid B ), respectively, and the total amount of six chlorogenic acids was 43.9-97.8 mg·g-1. The research results suggest that different varieties of S. rebaudiana cultivars contain different contents of chlorogenic acids, and the S. rebau-diana cultivars rich in chlorogenic acids can be used to develop and obtain chlorogenic acids. The resarch can provide a basis for expanding the development and utilization of S. rebaudiana. |
| Key words: Stevia rebaudiana, chlorogenic acids, leaves of Stevia rebaudiana cultivars, XAD-16 resin |
