| 摘要: |
| 采用反相HPLC法测定了广西梧州、龙州、苍梧、越南等多产地12批苦玄参药材指纹图谱,并对不同产地的苦玄参指纹图谱进行比较。色谱条件为:Luna C18(4.6×250 mm,5 μm)色谱柱,乙腈-水梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温25℃。结果12批苦玄参样品指纹图谱共标定了16个分离度良好的共有峰,方法的精密度、稳定性、重复性均符合国家相关规定,可作为控制苦玄参药材质量的定性标准。 |
| 关键词: 苦玄参 HPLC 指纹图谱 质量控制 |
| DOI： |
| 分类号:Q946 |
| Fund project: |
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| RP-HPLC fingerprint of Picria fel-terrae from South Guangxi |
|
LIANG Xiao-Yan, FANG Hong, NING De-Sheng,
CHEN Hai-Shan, HUANG Yong-Lin
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Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
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| Abstract: |
| The RP-HPLC assay was used to establish the fingerprint of Picria fel-terrae from South Guangxi,and the HPLC chromatogram of different origins of Picria fel-terrae were compared. The chromatography conditions were as follows:Luna C18 column(4.6×250 mm,5 μm),a mixture of acetonitrile and water as mobile phase in gradient mode,flow rate was 1.0 mL/min,detective wavelength at 254 nm,column temperature 25℃. The fingerprints of P.fel-terrae with 16 common peaks were determined. The RSD of precision and reproducibility lay within 5%. According to this method,the established fingerprint can be used for the identification and quality control of P.fel-terrae. |
| Key words: Picria fel-terrae HPLC fingerprint quality control |
