| 摘要: |
| 以考马斯亮蓝G250为染料,结合经典的凯氏定氮法测定结果,对影响染料结合法测定蛋白质含量的振荡时间、温度、考马斯亮蓝溶液浓度等因素进行了研究,并分析了由考马斯亮蓝染料和蛋白质结合后染料结合量(OD值差)与凯氏法测得的蛋白质百分含量之间的相关性。结果表明:测定的适宜条件是:温度15 ℃,处理时间50 min,考马斯亮蓝溶液的浓度是0.06 mg/mL。此条件形成的络合物较稳定,重复性较好,并且所测的染料结合量与凯氏定氮法测得的蛋白质含量间呈极显著的一元线性回归和相关关系。栽培甜荞和栽培苦荞的回归方程分别为:y=15.364x+3.865和y=10.769x+6.287,这两个回归方程差异显著,不能合并,分别适合于快速估计甜荞和苦荞种子的蛋白质含量。 |
| 关键词: 甜荞 苦荞 考马斯亮蓝 回归与相关 种子蛋白质 |
| DOI： |
| 分类号:Q946.1 |
| Fund project: |
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| Determination of the seed protein content on buckwheat by means of the dye method |
|
GUO Yu-Zhen, CHEN Qing-Fu*
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Institute of Plant Genetics and Breeding, School of Biological Technology and
Engineering, Guizhou Normal University, Guiyang 550001, China
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| Abstract: |
| Coomassie brilliant blue(CBB)G250 was used to determine the seed protein content of 25 common buckwheat accessions and 20 tartary buckwheat accessions. The results showed that the best conditions to determine the seed protein content of buckwheat with the dye combination method in this study were:15 ℃ 50 min and the dye concentration 0.06 mg/mL. Under these conditions,there are much significant linear regression and correlation between the dye combination amount and the protein content. The regression equations of the cultivated common buckwheat and tartary buckwheat are y=15.364x+3.865 and y=10.769x+6.287,respectively. There are much significant difference between the two equations,indicating that they fit to be used for calculation of the seed protein content on the cultivated common buckwheat and tartary buckwheat,respectively. |
| Key words: Common buckwheat Tartary buckwheat Coomassie brilliant blue Regression and correlation Seed protein |
