RP-HPLC测定红丝线提取物中紫蓝素的含量

Analysis of zilanine in extract of Peristrophe baphica by RP-HPLC

关键词:  高效液相色谱法  紫蓝素  红丝线提取物

Key words:  HPLC  zilanine  extract of Peristrophe baphica

摘要:

建立了红丝线提取物中紫蓝素的测定方法。采用反相高效液相色谱法,色谱柱为ZORBAX XDB-C18(4.6 mm×150 mm,5 μm); 流动相:V(乙腈):V[75 mmol/L乙酸铵+0.5 mmol/L EGTA(pH7.0)]=8:92; 流速:1 mL/min; 检测波长590 nm。紫蓝素的线性范围为2.5～50 mg/L(r=0.999 9),回收率97.9%～101.5%。该法简便、准确,重复性好,适用于测定红丝线提取物中紫蓝素的含量。

Abstract:

The paper established a RP-HPLC method for the determination of the content of zilanine in extract of Peristrophe baphica. RP-HPLC was conducted on a ZORBAX XDB-C18(4.6 mm×150 mm,5 μm)with CH₃CN-[CH₃COONH₄(75 mmol/L)+EGTA(0.5 mmol/L,pH=7.0)]=8:92; flow rate 1.0 mL/min,the detection wavelength was set at 590 nm. The linear range of zilanine was 2.5～50 mg/L(r=0.9999). The recoveries were 97.9%～101.5%. The method is simple and accurate with good reproducibility,it is suitable for the determination of zilanine content in extract of P.baphica.