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| 姜花属SRAP-PCR体系的优化与建立 |
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高丽霞1,3, 刘 念2*, 黄邦海3, 张 伟4
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1.中国科学院 华南植物园, 广州 510650;2.仲恺农业技术学院, 广州 510225;3.广州市
农业技术推广中心, 广州 510520;4.华南农业大学, 广州 510642
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| 摘要: |
| 对姜花属的SRAP-PCR反应体系进行了Mg离子、dNTPs、Taq酶、引物以及模板DNA浓度等方面的优化,除Mg离子浓度外,优化后的体系在其它各成分上,与原始体系相比,均显著降低了用量,节约了成本。并用优化后的体系对姜科其它14个属进行了扩增,均可得到良好的扩增效果,而且揭示的多态性很高,说明SRAP标记可以用来进行姜科各属内及属间分类和亲缘关系分析。 |
| 关键词: 姜花属 SRAP 优化 分类 亲缘关系分析 |
| DOI： |
| 分类号:Q949.71 |
| Fund project: |
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| Optimization and formation SRAP system in Hedychium |
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GAO Li-Xia1,3, LIU Nian2*, HUANG Bang-Hai3, ZHANG Wei4
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1.South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China;2.Zhongkai University
of Agriculture and Technology, Guangzhou 510225, China;3.Guangzhou Agricultural Technology Extension
Center, Guangzhou 510520, China;4.South China Agricultural University, Guangzhou 510642, China
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| Abstract: |
| The system of SRAP in Hedychium was optimized including the concentration of Mg2+,dNTPs,TaqE,primers and DNA template etc. Compared with original system,this system reduced notably the consumption of all components except Mg2+. Amplified reactions of other fourteen genera in Zingiberaceae with this system appeared clear bands and high polymorphism. The result showed that SRAP maker can be used in taxonomy and genetic connection analysis of varieties,species or genera in Zingiberaceae. |
| Key words: Hedychium SRAP optimize taxonomy genetic connection analysis |
