| 摘要: |
| 以灯盏花花葶、花盘及叶柄为外植体,MS为基本培养基,通过不同的激素种类和浓度配比,建立灯盏花组培快繁体系。结果如下:在所有实验方案中,花葶的出愈率最高,是理想的快速繁殖材料。较适宜的诱导愈伤组织的培养基为MS+BA1.0 mg/L+IBA0.05 mg/L+蔗糖3.0%,诱导不定芽的培养基为MS+BA2.0 mg/L+IAA1.0 mg/L+蔗糖3.0%或MS+Kt3.0+IAA0.5 mg/L+蔗糖3.0%,而根的诱导则是在1/2MS+NAA1.0Mg/L+蔗糖3.0%的培养基上进行。同时对组织培养过程中灯盏花植株再生的方式进行了讨论。 |
| 关键词: 灯盏花 愈伤组织 不定芽 生根 组织培养 |
| DOI： |
| 分类号:Q943.1 |
| Fund project: |
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| Tissue culture of medical plant Erigeron breviscapus |
|
HUANG Heng-Yu1, LI Li1,2*, DANG Cheng-Lin2
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1.Institute of Ecology, Jishou University, Jishou 416000, China;2.Institute of Ecology
and Geobotany, Yunnan University, Kunming 650091, China
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| Abstract: |
| The tissue culture and rapid proliferation techniques of Erigeron breviscapus were studied by using scape,floral disc and petiole as explants. The explants were cultivated in different MS media with different types and concentrations of plant hormones. The main results can be concluded as follows:the scape is the best material for the propagation among the three explants,scape,floral disc and petiole,as it has the highest inductivity,the best growth and it is easy to produce adventitious buds. The suitable phytohormone compositions to induce callus,adventitious buds and roots are MS+BA 1.0 mg/L+IBA 0.05 mg/L+sugar 3.0%,Ms+BA 2.0 mg/L+IAA 1.0 mg/L+sugar 3.0% or Ms+Kt 3.0 mg/L+IAA 0.5 mg/L+sugar 3.0%,and 1/2Ms+NAA 1.0 mg/L+sugar 3.0%,respectively. The mode of plant regeneration of E.breviscapus in tissue culture has also been investigated. |
| Key words: Erigeron breviscapus callus adventitious bud rooting tissue culture |
